TY - JOUR
T1 - Activation of microsomal guanylate cyclase by a cytotoxic polypeptide
T2 - Melittin
AU - Lad, Pushkaraj J.
AU - Thomas Shier, W.
PY - 1979/7/12
Y1 - 1979/7/12
N2 - Melittin, a 26 amino acid polypeptide, activated membrane-associated guanylate cyclase in a manner suggesting that membrane phospholipids play an important role in regulating the enzyme activity. Melittin was unique in that it activated only the particulate activity in a dose-dependent manner in the concentration range 15 to 200 μg/ml, and, unlike other known activators, solubilization of enzyme did not occur. The effects of melittin on guanylate cyclase showed a lag of two minutes and were not blocked by inhibitors of prostaglandin synthetase or phospholipase suggesting that the effect was not mediated by prostaglandin endoperoxides or phospholipase products but may be due to the ability of melittin to alter membrane lipid properties.
AB - Melittin, a 26 amino acid polypeptide, activated membrane-associated guanylate cyclase in a manner suggesting that membrane phospholipids play an important role in regulating the enzyme activity. Melittin was unique in that it activated only the particulate activity in a dose-dependent manner in the concentration range 15 to 200 μg/ml, and, unlike other known activators, solubilization of enzyme did not occur. The effects of melittin on guanylate cyclase showed a lag of two minutes and were not blocked by inhibitors of prostaglandin synthetase or phospholipase suggesting that the effect was not mediated by prostaglandin endoperoxides or phospholipase products but may be due to the ability of melittin to alter membrane lipid properties.
UR - http://www.scopus.com/inward/record.url?scp=0018428905&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018428905&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(79)90980-X
DO - 10.1016/0006-291X(79)90980-X
M3 - Article
C2 - 38790
AN - SCOPUS:0018428905
SN - 0006-291X
VL - 89
SP - 315
EP - 321
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -