Activities of antioxidant and redox enzymes in human normal hepatic and hepatoma cell lines

Yuk Young Lee, Hong Gyum Kim, Haeng Im Jung, Youn Hee Shin, Sung Min Hong, Eun Hee Park, Jae Hoon Sa, Chang Jin Lim

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

The cellular defense system (including glutathione, glutathione-related enzymes, antioxidant and redox enzymes) plays a crucial role in cell survival and growth in aerobic organisms. To understand its physiological role in tumor cells, the glutathione content and related enzyme activities in the human normal hepatic cell line, Chang and human hepatoma cell line, HepG2, were systematically measured and compared. Superoxide dismutase, catalase, and glutathione peroxidase activities are 2.8-, 4.3-, and 2.9-fold higher in HepG2 cells than in Chang cells. Total glutathione content is also about 1.4-fold higher in HepG2, which is supported by significant increases in γ-glutamylcysteine synthetase and glutathione synthetase activities. Two other glutathione-related enzymes, glutathione reductase and γ-glutamyltranspeptidase, are up-regulated in HepG2 cells. However, thioredoxin reductase and glutathione S-transferase activities are significantly lower in HepG2 cells. These results propose that defense-related enzymes are largely modulated in tumor cells, which might be linked to their growth and maintenance.

Original languageEnglish (US)
Pages (from-to)305-311
Number of pages7
JournalMolecules and cells
Volume14
Issue number2
StatePublished - Oct 1 2002

Keywords

  • Catalase
  • Chang
  • Glutathione
  • Glutathione Peroxidase
  • HepG2
  • Superoxide Dismutase
  • Thioredoxin Reductase

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