TY - JOUR
T1 - ADP-ribosyl cyclase and CD38 catalyze the synthesis of a calcium-mobilizing metabolite from NADP
AU - Aarhus, Robert
AU - Graeff, Richard M.
AU - Dickey, Deborah M.
AU - Walseth, Timothy F.
AU - Lee, Hon Cheung
PY - 1995/12/22
Y1 - 1995/12/22
N2 - ADP-ribosyl cyclase catalyzes the cyclization of NAD+ to produce cyclic ADP-ribose (cADPR), which is emerging as an endogenous regulator of the Ca2+-induced Ca2+ release mechanism in cells. CD38 is a lymphocyte differentiation antigen which has recently been shown to be a bifunctional enzyme that can synthesize cADPR from NAD+ as well as hydrolyze cADPR to ADP-ribose. In this study, we Show that both the cyclase and CD38 can also catalyze the exchange of the nicotinamide group of NADP+ with nicotinic acid (NA). The product is nicotinic acid adenine dinucleotide phosphate (NAADP+), a metabolite we have previously shown to be potent in Ca2+ mobilization (Lee, H. C., and Aarhus, R. (1995) J. Biol. Chem. 270, 2152-2157). The switch of the catalysis to the exchange reaction requires acidic pH and NA. The half-maximal effective concentration of NA is about 5 mM for both the cyclase and CD38. In the absence of NA or at neutral pH, the cyclase converts NADP+ to another metabolite, which is identified as cyclic ADP-ribose 2′-phosphate. Under the same conditions, CD38 converts NADP+ to ADP-ribose 2′-phosphate instead, which is the hydrolysis product of cyclic ADP-ribose 2′-phosphate. That two different products of ADP-ribosyl cyclase and CD38, cADPR and NAADP+, are both involved in Ca2+ mobilization suggests a crucial role of these enzymes in Ca2+ signaling.
AB - ADP-ribosyl cyclase catalyzes the cyclization of NAD+ to produce cyclic ADP-ribose (cADPR), which is emerging as an endogenous regulator of the Ca2+-induced Ca2+ release mechanism in cells. CD38 is a lymphocyte differentiation antigen which has recently been shown to be a bifunctional enzyme that can synthesize cADPR from NAD+ as well as hydrolyze cADPR to ADP-ribose. In this study, we Show that both the cyclase and CD38 can also catalyze the exchange of the nicotinamide group of NADP+ with nicotinic acid (NA). The product is nicotinic acid adenine dinucleotide phosphate (NAADP+), a metabolite we have previously shown to be potent in Ca2+ mobilization (Lee, H. C., and Aarhus, R. (1995) J. Biol. Chem. 270, 2152-2157). The switch of the catalysis to the exchange reaction requires acidic pH and NA. The half-maximal effective concentration of NA is about 5 mM for both the cyclase and CD38. In the absence of NA or at neutral pH, the cyclase converts NADP+ to another metabolite, which is identified as cyclic ADP-ribose 2′-phosphate. Under the same conditions, CD38 converts NADP+ to ADP-ribose 2′-phosphate instead, which is the hydrolysis product of cyclic ADP-ribose 2′-phosphate. That two different products of ADP-ribosyl cyclase and CD38, cADPR and NAADP+, are both involved in Ca2+ mobilization suggests a crucial role of these enzymes in Ca2+ signaling.
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U2 - 10.1074/jbc.270.51.30327
DO - 10.1074/jbc.270.51.30327
M3 - Article
C2 - 8530456
AN - SCOPUS:0029616337
SN - 0021-9258
VL - 270
SP - 30327
EP - 30333
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 51
ER -