Advanced Backcross Quantitative Trait Loci (QTL) Analysis of Oil Concentration and Oil Quality Traits in Peanut (Arachis hypogaea L.)

Jeffrey N. Wilson; Ratan Chopra; Michael R. Baring; Michael Gomez Selvaraj; Charles E. Simpson; Jennifer Chagoya; Mark D. Burow

doi:10.1007/s12042-016-9180-5

Advanced Backcross Quantitative Trait Loci (QTL) Analysis of Oil Concentration and Oil Quality Traits in Peanut (Arachis hypogaea L.)

Jeffrey N. Wilson, Ratan Chopra, Michael R. Baring, Michael Gomez Selvaraj, Charles E. Simpson, Jennifer Chagoya, Mark D. Burow

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Peanut seed oil is an important commodity worldwide and breeding efforts have been to improve both the quality and quantity of oil produced. Identifying sources of variation and elucidating the genetics of oil concentration and quality in peanut is essential to advancing the development of improved genotypes. The objective of this study was to discover QTLs for oil traits in an advanced backcross population derived from a cross between a wild-species derived amphidiploid, TxAG-6, and a cultivated genotype, Florunner. A BC₁F₁ population was developed for genetic mapping and an advanced backcross BC₃F₆ population was phenotyped in three environments and genotyped using SSR markers. Composite interval mapping results identified three genomic regions associated with oil concentration in a combined analysis. Marker PM36, associated with oil concentration and multiple fatty acids in this study, mapped directly to a HD-ZIP transcription factor in diploid Arachis genome sequences. For fatty acid concentrations, results suggested 17 QTLs identified in two or more environments, 15 of which were present across environments. Fourteen genomic regions on 13 linkage groups contained significant QTLs for more than one trait, suggesting that same genes or gene families are responsible for multiple phenotypes. QTLs and the genes identified in this study could be effective tools in marker-assisted breeding targeted at pyramiding seed oil alleles from wild-species while minimizing introgression of non-target chromatin.

Original language	English (US)
Journal	Tropical Plant Biology
Volume	10
Issue number	1
DOIs	https://doi.org/10.1007/s12042-016-9180-5
State	Published - Mar 1 2017
Externally published	Yes

Bibliographical note

Funding Information:
This work was funded by USDA/NIFA award TEX08835 to MDB, National Peanut Board award #329/TX-99 to MRB, MDB, and CES, and through support provided by the Office of Agriculture, Research and Policy, Bureau of Food Security, U.S. Agency for International Development, under the terms of Award No. AID-ECG-A-00-07-0001 to The University of Georgia as management entity for the U.S. Feed the Future Innovation Lab on Peanut Productivity and Mycotoxin Control. The opinions expressed herein are those of the author(s) and do not necessarily reflect the views of the U.S. Agency for International Development.

Publisher Copyright:
© 2016, Springer Science+Business Media New York.

Keywords

Advanced backcross QTL
Arachis hypogaea
Fatty acids
Genetic markers
Oil
Peanut
SSR

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title = "Advanced Backcross Quantitative Trait Loci (QTL) Analysis of Oil Concentration and Oil Quality Traits in Peanut (Arachis hypogaea L.)",

abstract = "Peanut seed oil is an important commodity worldwide and breeding efforts have been to improve both the quality and quantity of oil produced. Identifying sources of variation and elucidating the genetics of oil concentration and quality in peanut is essential to advancing the development of improved genotypes. The objective of this study was to discover QTLs for oil traits in an advanced backcross population derived from a cross between a wild-species derived amphidiploid, TxAG-6, and a cultivated genotype, Florunner. A BC1F1 population was developed for genetic mapping and an advanced backcross BC3F6 population was phenotyped in three environments and genotyped using SSR markers. Composite interval mapping results identified three genomic regions associated with oil concentration in a combined analysis. Marker PM36, associated with oil concentration and multiple fatty acids in this study, mapped directly to a HD-ZIP transcription factor in diploid Arachis genome sequences. For fatty acid concentrations, results suggested 17 QTLs identified in two or more environments, 15 of which were present across environments. Fourteen genomic regions on 13 linkage groups contained significant QTLs for more than one trait, suggesting that same genes or gene families are responsible for multiple phenotypes. QTLs and the genes identified in this study could be effective tools in marker-assisted breeding targeted at pyramiding seed oil alleles from wild-species while minimizing introgression of non-target chromatin.",

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author = "Wilson, {Jeffrey N.} and Ratan Chopra and Baring, {Michael R.} and Selvaraj, {Michael Gomez} and Simpson, {Charles E.} and Jennifer Chagoya and Burow, {Mark D.}",

note = "Funding Information: This work was funded by USDA/NIFA award TEX08835 to MDB, National Peanut Board award #329/TX-99 to MRB, MDB, and CES, and through support provided by the Office of Agriculture, Research and Policy, Bureau of Food Security, U.S. Agency for International Development, under the terms of Award No. AID-ECG-A-00-07-0001 to The University of Georgia as management entity for the U.S. Feed the Future Innovation Lab on Peanut Productivity and Mycotoxin Control. The opinions expressed herein are those of the author(s) and do not necessarily reflect the views of the U.S. Agency for International Development. Publisher Copyright: {\textcopyright} 2016, Springer Science+Business Media New York.",

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Advanced Backcross Quantitative Trait Loci (QTL) Analysis of Oil Concentration and Oil Quality Traits in Peanut (Arachis hypogaea L.)

Abstract

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