Alignment of collagen matrices using magnetic nanowires and magnetic barcode readout using first order reversal curves (FORC) (invited)

Anirudh Sharma, Michael D. DiVito, Daniel E. Shore, Andrew D. Block, Katie Pollock, Peter Solheid, Joshua M. Feinberg, Jaime Modiano, Cornelius H. Lam, Allison Hubel, Bethanie J.H. Stadler

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Collagen matrices are one form of artificial tissue that has applications in biomimetic organs or tumors, and in fundamental biology. Anatomical organs and tissues are often composed of aligned collagen, and in this study cross-linking nickel magnetic nanowires (MNWs) to collagen allowed a one-step bi-directional alignment of the collagen matrices when processed in a uniform magnetic field. These matrices were analyzed by differential interference contrast (DIC) microscopy, scanning electron microscopy (SEM) and polarized transmittance. The bi-directional alignment was also confirmed by plated, stained arachnoid cells from the blood-brain-barrier (BBB). Arachnoid cells are morphologically sensitive to their extracellular matrix (ECM) environment, and in this study, they were observed to spider out in two distinct directions as predicted by microscopy and transmittance. In fact, MNW-collagen matrices plated with arachnoid-cells are promising for future studies of artificial BBBs. Other cells (here osteosarcoma) have been observed to internalize MNWs, which leads to the possibility of barcoding matrices and cells with distinct signatures, pending a magnetic readout technique. To this aim, mixtures of two different MNW populations were analyzed using first order reversal curves (FORC), and the relative concentrations of the two populations were correctly estimated with negligible error for ratios of 1: 23 and only 7% error for ratios of 1: 115. Together, these studies open a path for magnetic identification of artificial tissues where distinct magnetic labels on matrices and in cells combine for a unique fingerprint.

Original languageEnglish (US)
Pages (from-to)176-181
Number of pages6
JournalJournal of Magnetism and Magnetic Materials
Volume459
DOIs
StatePublished - Aug 1 2018

Bibliographical note

Funding Information:
The authors acknowledge support from three UMN sources: Institute of Engineering in Medicine, MN Drive and MN Futures. Parts of this work were carried out in the UMN central facilities: University Imaging Center, Minnesota Nanofabrication Center , and the College of Science and Engineering Characterization Facility , which has received capital equipment funding from the NSF through the UMN MRSEC program under Award Numbers DMR-0819885 and DMR-1420013.

Publisher Copyright:
© 2017

Keywords

  • Arachnoid cells
  • Blood-brain-barrier
  • Collagen
  • FORC
  • Magnetic alignment
  • Magnetic nanowires
  • Nano barcodes
  • Osteosarcoma

How much support was provided by MRSEC?

  • Shared

Reporting period for MRSEC

  • Period 5

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