TY - JOUR
T1 - Alkaloids from the leaves of Uncaria rhynchophylla And Their Inhibitory Activity on NO production in lipopolysaccharide-activated microglia
AU - Yuan, Dan
AU - Ma, Bin
AU - Wu, Chunfu
AU - Yang, Jingyu
AU - Zhang, Lijia
AU - Liu, Suiku
AU - Wu, Lijun
AU - Kano, Yoshihiro
PY - 2008/7
Y1 - 2008/7
N2 - Two new isomeric alkaloids, 18,19-dehydrocorynoxinic acid B (1) and 18,19-dehydrocorynoxinic acid (2), were isolated from the CHCIj extract of the leaves of Uncaria rhynchophylla, together with four known rhynchophylline-type alkaloids, corynoxeine (3), isocorynoxeine (4), rhynchophylline (5), and isorhynchophylline (6), and an indole alkaloid glucoside, vincoside lactam (7). The structures of compounds 1 and 2 were elucidated by spectroscopic methods including UV, IR, HREIMS, 1D and 2D NMR, and CD experiments. The activity assay showed that compounds 3-6, with a C-16 carboxylic ester group, and 7 exhibited inhibitory activity on lipopolysaccharide (LPS)-induced NO release in primary cultured rat cortical microglia (IC50: 13.7-19.0 μM). However, only weak inhibitory activity was observed for compounds 1 and 2, with a C-16 carboxylic acid group (IC50: > 100 μM).
AB - Two new isomeric alkaloids, 18,19-dehydrocorynoxinic acid B (1) and 18,19-dehydrocorynoxinic acid (2), were isolated from the CHCIj extract of the leaves of Uncaria rhynchophylla, together with four known rhynchophylline-type alkaloids, corynoxeine (3), isocorynoxeine (4), rhynchophylline (5), and isorhynchophylline (6), and an indole alkaloid glucoside, vincoside lactam (7). The structures of compounds 1 and 2 were elucidated by spectroscopic methods including UV, IR, HREIMS, 1D and 2D NMR, and CD experiments. The activity assay showed that compounds 3-6, with a C-16 carboxylic ester group, and 7 exhibited inhibitory activity on lipopolysaccharide (LPS)-induced NO release in primary cultured rat cortical microglia (IC50: 13.7-19.0 μM). However, only weak inhibitory activity was observed for compounds 1 and 2, with a C-16 carboxylic acid group (IC50: > 100 μM).
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U2 - 10.1021/np8000305
DO - 10.1021/np8000305
M3 - Article
C2 - 18588343
AN - SCOPUS:49049114068
SN - 0163-3864
VL - 71
SP - 1271
EP - 1274
JO - Journal of Natural Products
JF - Journal of Natural Products
IS - 7
ER -