Alpha-actinin localization in the junctional complex of intestinal epithelial cells

S. W. Craig, J. V. Pardo

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

We have used antibody to chicken gizzard alpha-actinin to identify and localize this molecule in chicken intestinal epithelium. The antibody binds only to alpa-actinin when tested against a crude extract of chicken gizzard. Extracts of purified epithelial cells contain a molecule which has a subunit molecular weight of 100,000 on sodium dodecyl sulphate gels and which is able to inhibit the interaction of alpha-actinin antibody and 125I-labeled chicken gizzard alpha-actinin. By indirect immunofluorescence, alpha-actinin is localized in the apical portion of chicken intestinal epithelial cells. Ethanol-fixed cryostat sections of intestine taken through the apical portion of the epithelial cells and in a plane perpendicular to the long axis of the cells show that alpha-actinin is organized in a polygonal pattern which corresponds to the outlines of the polygonally packed epithelial cells.

Original languageEnglish (US)
Pages (from-to)203-210
Number of pages8
JournalJournal of Cell Biology
Volume80
Issue number1
DOIs
StatePublished - 1979

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