The metabolism of myeloma cells was altered to reduce lactate production in consecutive fed-batch cultures. The glucose concentration was maintained at low levels (0.28-0.55 mM) by employing a dynamic nutrient feeding method based on on-line measurement of the oxygen uptake rate (OUR) to estimate the metabolic demand of the cells. This strategy has been previously reported to be applied to cultures of hybridoma cells, in which the production of lactate was significantly reduced by thus maintaining the glucose concentration at low levels. However, for this cell line, a single fed-batch culture was not sufficient to alter the cellular metabolism, even at a glucose concentration of 0.28 mM. Two consecutive fed-batch cultures were employed to ensure that the cells were cultivated under a low glucose concentration for a sufficiently prolonged period of time to allow a switch of the cellular metabolism from a glycolytic (high lactate production) to oxidative (low lactate production) state.
Bibliographical noteFunding Information:
The support from Protein Design Laboratories Inc. is gratefully acknowledged. AFE was supported by a fellowship from the Department of Science and Technology, The Republic of the Philippines.
- Cell culture