Abstract
The effects of NaCl concentration on bleomycin-induced cleavages of single-strand and double-strand DNA fragments containing the phage G4 origin of complementary DNA Strand synthesis were investigated. It was found that bleomycin could be used as a reagent to analyze secondary and tertiary structures and subtle changes of DNA structures. The effects of NaCl concentration on cleavages of single-stranded DNA were distinct at every target site, indicating that the diversity of topolotical properties of DNA might change the selectivity of the bleomycin-induced DNA cleavage. These results showed alternative secondary structures within and close to the G4 origin of complementary DNA Strand synthesis.
Original language | English (US) |
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Pages (from-to) | 394-398 |
Number of pages | 5 |
Journal | Bioscience, Biotechnology and Biochemistry |
Volume | 56 |
Issue number | 3 |
DOIs | |
State | Published - 1992 |
Bibliographical note
Funding Information:Acknowledgments. This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan