1. 1. Islet amyloid isolated from the pancreas of a 20-year-old cougar (Felis concolor) was dissolved and purified by gel permeation and reversed phase HPLC mino acid sequence analysis. 2. 2. N-Terminal amino acid sequence analysis of the purified protein revealed a primary structure (positions 1-28) identical to islet amyloid polypeptide (IAPP) from domesticated cats. 3. 3. IAPP from the cougar, like IAPP from the human and domesticated cat, incorporates an inherently amyloidogenic AILS sequence at positions 25-28.
|Original language||English (US)|
|Number of pages||5|
|Journal||Comparative Biochemistry and Physiology -- Part B: Biochemistry and|
|State||Published - 1991|
Bibliographical noteFunding Information:
Acknowledgements--This work was supportedb y Grant ROI DK36734o f the National Institoutfe D iabeteas nd Digestivea nd Kidney Disease, the SweMdeisdhi calR e-search Council (Project5 N9o4.1 )t,h e Nordic Insulin Fund, and A. Louis-Hansen's Memorial Fund. The authors wish to thankK athyJ ordan,K athyB urg, and Christer Bergman for their technicaasls istance.