An acid sensing ion channel (ASIC) localizes to small primary afferent neurons in rats

Timothy H. Olson, Maureen S. Riedl, Lucy Vulchanova, Xilma R. Ortiz-Gonzalez, Robert Elde

Research output: Contribution to journalArticlepeer-review

122 Scopus citations

Abstract

THE acid sensing ion channel (ASIC) identified in rat brain and spinal cord is potentially involved in the transmission of acid-induced nociception. We have developed polyclonal antisera against ASIC, and used them to screen rat brain and spinal cord using immunocytochemistry. ASIC-immunoreactivity (- ir) is present in but not limited to the superficial dorsal horn, the dorsal root ganglia (DRG) and the spinal trigeminal nucleus, as well as peripheral nerve fibers. These observations, combined with the disappearance of ASIC-ir following dorsal rhizotomy, suggest localization of ASIC to primary afferents. DRG ASIC-ir co-localizes with substance P (SP) and calcitonin gene-related peptide (CGRP)-ir in small capsaicin-sensitive cell bodies, suggesting that ASIC is poised to play a role in the transduction of noxious stimuli.

Original languageEnglish (US)
Pages (from-to)1109-1113
Number of pages5
JournalNeuroreport
Volume9
Issue number6
DOIs
StatePublished - Apr 20 1998

Keywords

  • ASIC
  • BNaC
  • Co-localization
  • DRASIC
  • MDEG
  • Rat

Fingerprint Dive into the research topics of 'An acid sensing ion channel (ASIC) localizes to small primary afferent neurons in rats'. Together they form a unique fingerprint.

Cite this