An induced chromosomal translocation in soybean disrupts a KASI ortholog and is associated with a high-sucrose and low-oil seed phenotype

Austin A. Dobbels; Jean Michel Michno; Benjamin W. Campbell; Kamaldeep S. Virdi; Adrian O. Stec; Gary J. Muehlbauer; Seth L. Naeve; Robert M. Stupar

doi:10.1534/g3.116.038596

An induced chromosomal translocation in soybean disrupts a KASI ortholog and is associated with a high-sucrose and low-oil seed phenotype

Austin A. Dobbels, Jean Michel Michno, Benjamin W. Campbell, Kamaldeep S. Virdi, Adrian O. Stec, Gary J. Muehlbauer, Seth L. Naeve, Robert M. Stupar

Agronomy and Plant Genetics

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Mutagenesis is a useful tool in many crop species to induce heritable genetic variability for trait improvement and gene discovery. In this study, forward screening of a soybean fast neutron (FN) mutant population identified an individual that produced seed with nearly twice the amount of sucrose (8.1% on dry matter basis) and less than half the amount of oil (8.5% on dry matter basis) as compared to wild type. Bulked segregant analysis (BSA), comparative genomic hybridization, and genome resequencing were used to associate the seed composition phenotype with a reciprocal translocation between chromosomes 8 and 13. In a backcross population, the translocation perfectly cosegregated with the seed composition phenotype and exhibited non-Mendelian segregation patterns. We hypothesize that the translocation is responsible for the altered seed composition by disrupting a β-ketoacyl-[acyl carrier protein] synthase 1 (KASI) ortholog. KASI is a core fatty acid synthesis enzyme that is involved in the conversion of sucrose into oil in developing seeds. This finding may lead to new research directions for developing soybean cultivars with modified carbohydrate and oil seed composition.

Original language	English (US)
Pages (from-to)	1215-1223
Number of pages	9
Journal	G3: Genes, Genomes, Genetics
Volume	7
Issue number	4
DOIs	https://doi.org/10.1534/g3.116.038596
State	Published - Apr 1 2017

Bibliographical note

Funding Information:
The authors are grateful to Dimitri von Ruckert, Jill Miller-Garvin, and Jeffrey Roessler for project support, and acknowledge the Minnesota Supercomputing Institute at the University of Minnesota for providing resources that contributed to the research results reported within this paper (URL: http://www.msi.umn.edu). This work was supported by the Minnesota Soybean Research and Promotion Council (#19-16C), the United Soybean Board (#1520-532-5603), and the National Science Foundation (#MCB-1444581). Author contributions: A.A.D., R.M.S., S.L.N., and G.J.M. conceived this study. A.A.D., J.-M.M., B.W.C., K.S.V., and A.O.S. performed the research. A.A.D., S.L.N., and R.M.S. wrote the manuscript.

Publisher Copyright:
© 2017 Dobbels et al.

Keywords

Fast neutron
Oil
Soybean
Sucrose
Translocation

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title = "An induced chromosomal translocation in soybean disrupts a KASI ortholog and is associated with a high-sucrose and low-oil seed phenotype",

abstract = "Mutagenesis is a useful tool in many crop species to induce heritable genetic variability for trait improvement and gene discovery. In this study, forward screening of a soybean fast neutron (FN) mutant population identified an individual that produced seed with nearly twice the amount of sucrose (8.1% on dry matter basis) and less than half the amount of oil (8.5% on dry matter basis) as compared to wild type. Bulked segregant analysis (BSA), comparative genomic hybridization, and genome resequencing were used to associate the seed composition phenotype with a reciprocal translocation between chromosomes 8 and 13. In a backcross population, the translocation perfectly cosegregated with the seed composition phenotype and exhibited non-Mendelian segregation patterns. We hypothesize that the translocation is responsible for the altered seed composition by disrupting a β-ketoacyl-[acyl carrier protein] synthase 1 (KASI) ortholog. KASI is a core fatty acid synthesis enzyme that is involved in the conversion of sucrose into oil in developing seeds. This finding may lead to new research directions for developing soybean cultivars with modified carbohydrate and oil seed composition.",

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note = "Funding Information: The authors are grateful to Dimitri von Ruckert, Jill Miller-Garvin, and Jeffrey Roessler for project support, and acknowledge the Minnesota Supercomputing Institute at the University of Minnesota for providing resources that contributed to the research results reported within this paper (URL: http://www.msi.umn.edu). This work was supported by the Minnesota Soybean Research and Promotion Council (#19-16C), the United Soybean Board (#1520-532-5603), and the National Science Foundation (#MCB-1444581). Author contributions: A.A.D., R.M.S., S.L.N., and G.J.M. conceived this study. A.A.D., J.-M.M., B.W.C., K.S.V., and A.O.S. performed the research. A.A.D., S.L.N., and R.M.S. wrote the manuscript. Publisher Copyright: {\textcopyright} 2017 Dobbels et al.",

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AU - Michno, Jean Michel

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AU - Virdi, Kamaldeep S.

AU - Stec, Adrian O.

AU - Muehlbauer, Gary J.

AU - Naeve, Seth L.

AU - Stupar, Robert M.

N1 - Funding Information: The authors are grateful to Dimitri von Ruckert, Jill Miller-Garvin, and Jeffrey Roessler for project support, and acknowledge the Minnesota Supercomputing Institute at the University of Minnesota for providing resources that contributed to the research results reported within this paper (URL: http://www.msi.umn.edu). This work was supported by the Minnesota Soybean Research and Promotion Council (#19-16C), the United Soybean Board (#1520-532-5603), and the National Science Foundation (#MCB-1444581). Author contributions: A.A.D., R.M.S., S.L.N., and G.J.M. conceived this study. A.A.D., J.-M.M., B.W.C., K.S.V., and A.O.S. performed the research. A.A.D., S.L.N., and R.M.S. wrote the manuscript. Publisher Copyright: © 2017 Dobbels et al.

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N2 - Mutagenesis is a useful tool in many crop species to induce heritable genetic variability for trait improvement and gene discovery. In this study, forward screening of a soybean fast neutron (FN) mutant population identified an individual that produced seed with nearly twice the amount of sucrose (8.1% on dry matter basis) and less than half the amount of oil (8.5% on dry matter basis) as compared to wild type. Bulked segregant analysis (BSA), comparative genomic hybridization, and genome resequencing were used to associate the seed composition phenotype with a reciprocal translocation between chromosomes 8 and 13. In a backcross population, the translocation perfectly cosegregated with the seed composition phenotype and exhibited non-Mendelian segregation patterns. We hypothesize that the translocation is responsible for the altered seed composition by disrupting a β-ketoacyl-[acyl carrier protein] synthase 1 (KASI) ortholog. KASI is a core fatty acid synthesis enzyme that is involved in the conversion of sucrose into oil in developing seeds. This finding may lead to new research directions for developing soybean cultivars with modified carbohydrate and oil seed composition.

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KW - Fast neutron

KW - Oil

KW - Soybean

KW - Sucrose

KW - Translocation

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JO - G3: Genes, Genomes, Genetics

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ER -

An induced chromosomal translocation in soybean disrupts a KASI ortholog and is associated with a high-sucrose and low-oil seed phenotype

Abstract

Bibliographical note

Keywords

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