Abstract
Gas chromatography-mass spectrometry-selected-ion monitoring provided a simple and sensitive method for analyzing amino acids in plasma and brain samples. Although the sensitivities of chemical ionization and electron-impact ionization were similar chemical ionization produced higher-mass ions, which might increase the selectivity of the assay. Both chemical and electron-impact ionization distinguished the natural amino acids from the 15N-labelled amino acids. The recovery of amino acids from plasma and brain samples was ca. 75%. The amino acid levels determined by gas chromatography-mass spectrometry were comparable with the amino acid levels determined by high-performance liquid chromatography or amino acid analyzer.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 245-255 |
| Number of pages | 11 |
| Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
| Volume | 425 |
| Issue number | C |
| DOIs | |
| State | Published - 1988 |
Bibliographical note
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