Analysis of deoxyribonucleic acid distribution in noncleaving oocytes from patients undergoing in vitro fertilization

M. G. O'Rand; B. Herman; J. Diguiseppi; J. Halme; M. G. Hammond; L. M. Talbert

doi:10.1016/S0015-0282(16)49585-3

Analysis of deoxyribonucleic acid distribution in noncleaving oocytes from patients undergoing in vitro fertilization

M. G. O'Rand, B. Herman, J. Diguiseppi, J. Halme, M. G. Hammond, L. M. Talbert

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The purpose of this study was to describe the quantity and distribution of deoxyribonucleic acid (DNA) in oocytes that did not fertilize or did fertilize and failed to cleave, from patients who underwent in vitro fertilization. Patients were selected with at least one cleaving egg, so that the sperm population was known to be fertile, and failure of fertilization or cleavage in the remaining oocytes could be attributed to nonspermatozoan factors. The noncleaving oocytes were classified into five categories, the majority of which (71%) lacked a polar body and any morphologically identifiable nucleus or germinal vesicle. Three general defects were found: (1) failure to replicate the DNA properly; (2) failure to package the DNA properly; and (3) failure to organize the nuclear material properly after sperm penetration. It is concluded that either altered stimulation protocols or altered in vitro maturation conditions are needed to increase the average number of normal embryos available for transfer.

Original language	English (US)
Pages (from-to)	452-460
Number of pages	9
Journal	Fertility and Sterility
Volume	46
Issue number	3
DOIs	https://doi.org/10.1016/S0015-0282(16)49585-3
State	Published - Jan 1 1986

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abstract = "The purpose of this study was to describe the quantity and distribution of deoxyribonucleic acid (DNA) in oocytes that did not fertilize or did fertilize and failed to cleave, from patients who underwent in vitro fertilization. Patients were selected with at least one cleaving egg, so that the sperm population was known to be fertile, and failure of fertilization or cleavage in the remaining oocytes could be attributed to nonspermatozoan factors. The noncleaving oocytes were classified into five categories, the majority of which (71%) lacked a polar body and any morphologically identifiable nucleus or germinal vesicle. Three general defects were found: (1) failure to replicate the DNA properly; (2) failure to package the DNA properly; and (3) failure to organize the nuclear material properly after sperm penetration. It is concluded that either altered stimulation protocols or altered in vitro maturation conditions are needed to increase the average number of normal embryos available for transfer.",

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AU - Herman, B.

AU - Diguiseppi, J.

AU - Halme, J.

AU - Hammond, M. G.

AU - Talbert, L. M.

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N2 - The purpose of this study was to describe the quantity and distribution of deoxyribonucleic acid (DNA) in oocytes that did not fertilize or did fertilize and failed to cleave, from patients who underwent in vitro fertilization. Patients were selected with at least one cleaving egg, so that the sperm population was known to be fertile, and failure of fertilization or cleavage in the remaining oocytes could be attributed to nonspermatozoan factors. The noncleaving oocytes were classified into five categories, the majority of which (71%) lacked a polar body and any morphologically identifiable nucleus or germinal vesicle. Three general defects were found: (1) failure to replicate the DNA properly; (2) failure to package the DNA properly; and (3) failure to organize the nuclear material properly after sperm penetration. It is concluded that either altered stimulation protocols or altered in vitro maturation conditions are needed to increase the average number of normal embryos available for transfer.

AB - The purpose of this study was to describe the quantity and distribution of deoxyribonucleic acid (DNA) in oocytes that did not fertilize or did fertilize and failed to cleave, from patients who underwent in vitro fertilization. Patients were selected with at least one cleaving egg, so that the sperm population was known to be fertile, and failure of fertilization or cleavage in the remaining oocytes could be attributed to nonspermatozoan factors. The noncleaving oocytes were classified into five categories, the majority of which (71%) lacked a polar body and any morphologically identifiable nucleus or germinal vesicle. Three general defects were found: (1) failure to replicate the DNA properly; (2) failure to package the DNA properly; and (3) failure to organize the nuclear material properly after sperm penetration. It is concluded that either altered stimulation protocols or altered in vitro maturation conditions are needed to increase the average number of normal embryos available for transfer.

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Analysis of deoxyribonucleic acid distribution in noncleaving oocytes from patients undergoing in vitro fertilization

Abstract

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