Abstract
Fetal bone marrow B lineage cells representing multiple stages of B cell development were isolated by two-color cell sorting and analyzed for immunoglobulin H and T-cell receptor (TCR) gamma and delta gene rearrangements. Analysis of CD10+/surface mu- cells using a JH probe revealed a high frequency of rearrangements; some of these rearrangements used the 3′ D region gene DQ52. Analysis of CD10+/surface mu- cells revealed no detectable TCR-γ or -δ rearrangements, nor were TCR-δ rearrangements detected in CD10+/surface mu- cells, despite the limited repertoire of these genes. These observations are surprising given the high frequency of TCR δ/γ rearrangements in B cell precursor acute lymphoblastic leukemia, and identify a potential difference in patterns of gene rearrangement that distinguish normal and leukemic B cell precursors.
Original language | English (US) |
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Pages (from-to) | 1196-1200 |
Number of pages | 5 |
Journal | Blood |
Volume | 76 |
Issue number | 6 |
State | Published - Sep 15 1990 |