Analysis of nevirapine resistance mutations in cloned HIV type 1 variants from HIV-infected Ugandan infants using a single-step amplification-sequencing method (AmpliSeq)

William Ian Towler; Jessica D. Church; James R. Eshleman; Mary Glenn Fowler; Laura A. Guay; J. Brooks Jackson; Susan H. Eshleman

doi:10.1089/aid.2008.0109

Analysis of nevirapine resistance mutations in cloned HIV type 1 variants from HIV-infected Ugandan infants using a single-step amplification-sequencing method (AmpliSeq)

William Ian Towler, Jessica D. Church, James R. Eshleman, Mary Glenn Fowler, Laura A. Guay, J. Brooks Jackson, Susan H. Eshleman

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4 Scopus citations

Abstract

We analyzed the genetic linkage of nevirapine (NVP) resistance mutations and the genetic complexity of HIV-1 variants in Ugandan infants who were HIV infected despite single dose (SD) prophylaxis. Plasma samples were obtained from six HIV-infected infants who had two or more NVP resistance mutations detected by population sequencing (ViroSeq). ViroSeq PCR products were cloned and transformed, and a single-step amplification-sequencing reaction (AmpliSeq) was used to analyze NVP resistance mutations in cloned HIV-1 variants directly from bacterial colonies. Fifty clones were analyzed for each infant sample. This analysis revealed numerous NVP resistance mutations not detected by population sequencing, genetically linked NVP resistance mutations, and a high degree of genetic complexity at codons that influence NVP susceptibility.

Original language	English (US)
Pages (from-to)	1209-1213
Number of pages	5
Journal	AIDS Research and Human Retroviruses
Volume	24
Issue number	9
DOIs	https://doi.org/10.1089/aid.2008.0109
State	Published - Sep 1 2008
Externally published	Yes

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Towler, W. I., Church, J. D., Eshleman, J. R., Fowler, M. G., Guay, L. A., Jackson, J. B., & Eshleman, S. H. (2008). Analysis of nevirapine resistance mutations in cloned HIV type 1 variants from HIV-infected Ugandan infants using a single-step amplification-sequencing method (AmpliSeq). AIDS Research and Human Retroviruses, 24(9), 1209-1213. https://doi.org/10.1089/aid.2008.0109

Analysis of nevirapine resistance mutations in cloned HIV type 1 variants from HIV-infected Ugandan infants using a single-step amplification-sequencing method (AmpliSeq). / Towler, William Ian; Church, Jessica D.; Eshleman, James R. et al.
In: AIDS Research and Human Retroviruses, Vol. 24, No. 9, 01.09.2008, p. 1209-1213.

Research output: Contribution to journal › Article › peer-review

Towler, WI, Church, JD, Eshleman, JR, Fowler, MG, Guay, LA, Jackson, JB & Eshleman, SH 2008, 'Analysis of nevirapine resistance mutations in cloned HIV type 1 variants from HIV-infected Ugandan infants using a single-step amplification-sequencing method (AmpliSeq)', AIDS Research and Human Retroviruses, vol. 24, no. 9, pp. 1209-1213. https://doi.org/10.1089/aid.2008.0109

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Analysis of nevirapine resistance mutations in cloned HIV type 1 variants from HIV-infected Ugandan infants using a single-step amplification-sequencing method (AmpliSeq)

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