Auxins are a group of naturally occurring phytohormones that have numerous functions in plant growth and development. The identification and quantification of these hormones from plant tissues has a long and varied history, dating back as far as the mid-19th century. The evolution of auxin analysis closely mirrors the evolution of analytical chemistry. However, despite the fact that the methods for auxin determination have evolved since the initial bioassays, few of the techniques developed over the years have been completely abandoned, and many of them are still used in routine auxin analyses today.
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