Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions

Ryota Sakamoto; Vincent Noireaux; Yusuke T. Maeda

doi:10.1038/s41598-018-25532-3

Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions

Ryota Sakamoto, Vincent Noireaux, Yusuke T. Maeda

Physics and Astronomy (Twin Cities)

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Cellular surface breaks the symmetry of molecular diffusion across membrane. Here, we study how steric interactions between the surface and the bulk of cell-sized emulsion droplets alters gene expression emulated by a cell-free transcription/translation (TXTL) system. The concentration of synthesized reporter proteins in droplets of radius R shows an anomalous geometric scaling of R⁴ different from the expected size-dependence of R³. Given that TXTL becomes less efficient at thin surface layer, a mathematical model explains the anomalous size-dependence found in experiment. The surface of cell-sized compartment can thus play a regulatory role for cell-free gene expression.

Original language	English (US)
Article number	7364
Journal	Scientific reports
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41598-018-25532-3
State	Published - Dec 1 2018

Bibliographical note

Funding Information:
We thank Z. Izri for fruitfull discussion. This work was supported by Human Frontier Science Program Research Grant (RGP0037/2015) and JSPS KAKENHI (16H00805 Synergy of Structure and Fluctuation, 17H05234 Hadean Bioscience, and 17KT0025 Grant-in-Aid for Scientific Research (B) 17KT0025) from MEXT.

Publisher Copyright:
© 2018 The Author(s).

Access

10.1038/s41598-018-25532-3

OpenUrl availability

Full text

Cite this

@article{7ad52cdeb2af4745abd636fbb82e163b,

title = "Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions",

abstract = "Cellular surface breaks the symmetry of molecular diffusion across membrane. Here, we study how steric interactions between the surface and the bulk of cell-sized emulsion droplets alters gene expression emulated by a cell-free transcription/translation (TXTL) system. The concentration of synthesized reporter proteins in droplets of radius R shows an anomalous geometric scaling of R4 different from the expected size-dependence of R3. Given that TXTL becomes less efficient at thin surface layer, a mathematical model explains the anomalous size-dependence found in experiment. The surface of cell-sized compartment can thus play a regulatory role for cell-free gene expression.",

author = "Ryota Sakamoto and Vincent Noireaux and Maeda, {Yusuke T.}",

note = "Funding Information: We thank Z. Izri for fruitfull discussion. This work was supported by Human Frontier Science Program Research Grant (RGP0037/2015) and JSPS KAKENHI (16H00805 Synergy of Structure and Fluctuation, 17H05234 Hadean Bioscience, and 17KT0025 Grant-in-Aid for Scientific Research (B) 17KT0025) from MEXT. Publisher Copyright: {\textcopyright} 2018 The Author(s).",

year = "2018",

month = dec,

day = "1",

doi = "10.1038/s41598-018-25532-3",

language = "English (US)",

volume = "8",

journal = "Scientific reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

number = "1",

}

TY - JOUR

T1 - Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions

AU - Sakamoto, Ryota

AU - Noireaux, Vincent

AU - Maeda, Yusuke T.

N1 - Funding Information: We thank Z. Izri for fruitfull discussion. This work was supported by Human Frontier Science Program Research Grant (RGP0037/2015) and JSPS KAKENHI (16H00805 Synergy of Structure and Fluctuation, 17H05234 Hadean Bioscience, and 17KT0025 Grant-in-Aid for Scientific Research (B) 17KT0025) from MEXT. Publisher Copyright: © 2018 The Author(s).

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Cellular surface breaks the symmetry of molecular diffusion across membrane. Here, we study how steric interactions between the surface and the bulk of cell-sized emulsion droplets alters gene expression emulated by a cell-free transcription/translation (TXTL) system. The concentration of synthesized reporter proteins in droplets of radius R shows an anomalous geometric scaling of R4 different from the expected size-dependence of R3. Given that TXTL becomes less efficient at thin surface layer, a mathematical model explains the anomalous size-dependence found in experiment. The surface of cell-sized compartment can thus play a regulatory role for cell-free gene expression.

AB - Cellular surface breaks the symmetry of molecular diffusion across membrane. Here, we study how steric interactions between the surface and the bulk of cell-sized emulsion droplets alters gene expression emulated by a cell-free transcription/translation (TXTL) system. The concentration of synthesized reporter proteins in droplets of radius R shows an anomalous geometric scaling of R4 different from the expected size-dependence of R3. Given that TXTL becomes less efficient at thin surface layer, a mathematical model explains the anomalous size-dependence found in experiment. The surface of cell-sized compartment can thus play a regulatory role for cell-free gene expression.

UR - http://www.scopus.com/inward/record.url?scp=85046845649&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85046845649&partnerID=8YFLogxK

U2 - 10.1038/s41598-018-25532-3

DO - 10.1038/s41598-018-25532-3

M3 - Article

C2 - 29743508

AN - SCOPUS:85046845649

SN - 2045-2322

VL - 8

JO - Scientific reports

JF - Scientific reports

IS - 1

M1 - 7364

ER -

Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions

Abstract

Bibliographical note

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this