Copper nanoparticles (NCu) were synthetized and added to commercial glass ionomer cement, to evaluate in vitro its antibacterial activity against oral cavity strains. The NCu were synthesized by copper acetate reduction with L-ascorbic acid and characterized by FTIR, Raman, XPS, XRD and TEM. Then, commercial glass ionomer cement (GIC) was modified (MGIC) with various concentrations of NCu and physicochemically characterized. Cell viability was tested against human dental pulp fibroblasts (HDPFs) by Alamar-Blue assay and antibacterial test was performed against S. mutans and S. sanguinis by colony forming unit (CFU) growth method. Synthesized NCu rendered a mixture of both metallic copper and cuprous oxide (Cu2O). HDPF viability reduces with exposure time to the extracts (68–72% viability) and MGIC with 2–4 wt% NCu showed antimicrobial activity against the two tested strains.
Bibliographical noteFunding Information:
This work was supported by the National Council of Science and Technology (CONACYT) [grants projects 248378, 1360, and 268595] and PhD scholarship 483616, also from CONACYT. To the Nano Materials Laboratory, CINVESTAV-IPN, Merida, Yucatan, FOMIX-Yucatan 2008-108160, CONACYT LAB-2009-01-123913, CONACYT No: 188345 y 204822. We also thank Daniel Hector Aguilar Treviño and Wilian Cauich Ruiz for technical assistance in XRD and XPS experiments.
- Anaerobia bacteria
- Dental cement
- Metal nanoparticle
PubMed: MeSH publication types
- Journal Article