Antibiotic-inducible promoter regulated by the cell envelope stress-sensing two-component system LiaRS of Bacillus subtilis

Thorsten Mascher, Sara L. Zimmer, Terry Ann Smith, John D. Helmann

Research output: Contribution to journalArticlepeer-review

175 Scopus citations

Abstract

Soil bacteria are among the most prodigious producers of antibiotics. The Bacillus subtilis LiaRS (formerly YvqCE) two-component system is one of several antibiotic-sensing systems that coordinate the genetic response to cell wall-active antibiotics. Upon the addition of vancomycin or bacitracin, LiaRS autoregulates the liaIHGFSR operon. We have characterized the promoter of the lia operon and defined the cis-acting sequences necessary for antibiotic-inducible gene expression. A survey for compounds that act as inducers of the lia promoter revealed that it responds strongly to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin, and vancomycin). Chemicals that perturb the cytoplasmic membrane, such as organic solvents, are also weak inducers. Thus, the reporter derived from PliaI (the liaI promoter) provides a tool for the detection and classification of antimicrobial compounds.

Original languageEnglish (US)
Pages (from-to)2888-2896
Number of pages9
JournalAntimicrobial agents and chemotherapy
Volume48
Issue number8
DOIs
StatePublished - Aug 2004

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