Antimalarial drugs impact chemical messenger secretion by blood platelets

Kang Xiong-Hang, Jiayi He, Kaila Kemnetz-Ness, Christy Haynes

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Advances in antimalarial drug development are important for combating malaria. Among the currently identified antimalarial drugs, it is suggested that some interact directly with the malarial parasites while others interact indirectly with the parasites. While this approach leads to parasite elimination, little is known about how these antimalarial drugs impact immune cells that are also critical in malarial response. Methods: Herein, the effects of two common antimalarial drugs, chloroquine and quinine, on platelets were explored at both the bulk level, using high performance liquid chromatography, and the single cell level, using carbon-fiber microelectrode amperometry, to characterize any changes in chemical messenger secretion. Results: The data reveal that both drugs cause platelet activation and reduce the number of platelet exocytosis events as well as delay fusion pore opening and closing. Conclusions: This work demonstrates how chloroquine and quinine quantitatively and qualitatively impact in vitro platelet function. General significance: Overall, the goal of this work is to promote understanding about how antimalarial drugs impact platelets as this may affect antimalarial drug development as well as therapeutic approaches to treat malarial infection.

Original languageEnglish (US)
Article number100758
JournalBiochemistry and Biophysics Reports
Volume22
DOIs
StatePublished - Jul 2020

Bibliographical note

Funding Information:
This work was funded by the Biotechnology Training Grant NIH T32GM008347 to KX and support from the University of Minnesota's Undergraduate Research Opportunities Program to KK . The authors thank Amani Lee for his help in the discussion of Gaussian fitting.

Publisher Copyright:
© 2020

Keywords

  • Antimalarial drugs
  • Carbon-fiber microelectrode amperometry
  • Electrochemistry
  • Exocytosis
  • High-performance liquid chromatography
  • Platelets

PubMed: MeSH publication types

  • Journal Article

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