Antisense expression of the 20-hydroxyecdysone receptor (EcR) in transfected mosquito cells uncovers a new EcR isoform that varies at the C-terminal end

G. Jayachandran, A. M. Fallon

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The insect steroid hormone 20-hydroxyecdysone initiates a cascade of regulatory events in a temporal and tissuespecific manner by first binding to a complex of an ecdysone receptor (EcR) protein and a ultraspiracle protein. Using an antisense (As) ribonucleic acid approach, we show that disruption of EcR expression in transfected C7-10 cells from the mosquito Aedes albopictus affects survival and growth. From stably transfected cells, we recovered a new isoform of A. albopictus AalEcRa, which is named AalEcRb. The deduced amino acid sequence of AalEcRb was almost identical to that of AalEcRa, with the exception of a seven amino acid sequence near the C-terminus. Using polymerase chain reaction followed by restriction enzyme analysis, we found that AalEcRa is the predominant species expressed by wild-type C7-10 cells, while cells transfected with As-EcR expressed both isoforms at approximately equal levels.

Original languageEnglish (US)
Pages (from-to)522-529
Number of pages8
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume37
Issue number8
DOIs
StatePublished - Oct 27 2001

Keywords

  • Aedes albopictus
  • Antisense ribonucleic acid
  • C7-10 cell line
  • Ecdysone receptor
  • Transfection

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