Application and evaluation of anti-notch antibodies to modulate notch signaling

Wendy R. Gordon, Jon C. Aster

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

In recent years, several groups have reported the development of antibodies that inhibit or activate Notch signaling. Modulatory antibodies are valuable experimental tools that permit specific targeting of individual Notch receptor homologs (in contrast to pan-Notch-receptor inhibitors like gamma-secretase inhibitors), and show promise as therapeutic agents. Typically, Notch responsive luciferase reporter assays are used to validate and characterize modulatory antibodies. We describe detailed methods for performing dual luciferase-based signaling assays to read out modulation of Notch activity by antibodies designed to inhibit/activate signaling.

Original languageEnglish (US)
Title of host publicationNotch Signaling
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages323-333
Number of pages11
ISBN (Print)9781493911387
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1187
ISSN (Print)1064-3745

Keywords

  • Activating antibody
  • Inhibitory antibody
  • Luciferase assay
  • NRR
  • Notch

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