Spin labeled drugs can be utilized for immunoassays and determining the fraction of free drug in serum. To improve the sensitivity we have synthesized triacetonamine‐15N‐d17 by cyclization of phorone‐d14 with 15ND3. Phorone‐d14 was obtained by condensation of acetone‐d6. Triacetonamine‐15N‐d17 gave Tempone‐15N‐d16, a nitroxide with just two absorption peaks on the ESR spectrum, whereas Tempone gives 3 peaks. The contribution to line width of methylene and methy1 hydrogens of Tempone is evaluated by appropriate substitution of protium with deuterium.
|Original language||English (US)|
|Number of pages||9|
|Journal||Journal of Labelled Compounds and Radiopharmaceuticals|
|State||Published - Aug 1981|
- Drug Spin Labels