Applications of SPR for the characterization of molecules important in the pathogenesis and treatment of neurodegenerative diseases

Nathan J. Wittenberg, Bharath Wootla, Luke R. Jordan, Aleksandar Denic, Arthur E. Warrington, Sang Hyun Oh, Moses Rodriguez

Research output: Contribution to journalReview articlepeer-review

14 Scopus citations

Abstract

Characterization of binding kinetics and affinity between a potential drug and its receptor are key steps in the development of new drugs. Among the techniques available to determine binding affinities, surface plasmon resonance has emerged as the gold standard because it can measure binding and dissociation rates in real-time in a label-free fashion. Surface plasmon resonance is now finding applications in the characterization of molecules for treatment of neurodegenerative diseases, characterization of molecules associated with pathogenesis of neurodegenerative diseases and detection of neurodegenerative disease biomarkers. In addition it has been used in the characterization of a new class of natural autoantibodies that have therapeutic potential in a number of neurologic diseases. In this review we will introduce surface plasmon resonance and describe some applications of the technique that pertain to neurodegenerative disorders and their treatment.

Original languageEnglish (US)
Pages (from-to)449-463
Number of pages15
JournalExpert Review of Neurotherapeutics
Volume14
Issue number4
DOIs
StatePublished - Apr 2014

Bibliographical note

Funding Information:
This work was supported by grants from the NIH (R01 GM092993, R01 NS048357 and R21 NS073684) and the National Multiple Sclerosis Society (CA 1060A). This work was also supported by a High-Impact Pilot and Feasibility Award (HIPFA) and Novel Methodology Award (NMDA) from the Mayo Clinic Center for Translational Science Activities (CTSA) and Mayo Clinic CTSA grant number UL1 TR000135 from the National Center for Advancing Translational Science (NCATS), a component of the National Institutes of Health (NIH). We also acknowledge with thanks support from the Applebaum, Hilton, Peterson and Sanford Foundations, the Minnesota Partnership Award for Biotechnology and Medical Genomics, the Moon and Marilyn Park Directorship Fund and the McNeilus family.

Keywords

  • Alzheimer's disease
  • Amyloid-beta
  • Antibodies
  • Biomarkers
  • Lipid bilayer membrane
  • Multiple sclerosis
  • Neuromyelitis optica
  • Surface modification
  • Surface plasmon resonance

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