Arachidonic acid metabolites produced by bovine alveolar macrophages.

M. G. O'Sullivan, R. T. Dobrowsky, L. N. Fleisher, N. C. Olson, T. T. Brown

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Bovine alveolar macrophages, obtained by bronchoalveolar lavage, were labeled with tritiated arachidonic acid. The cells were stimulated with calcium ionophore A23187, and the radiolabeled arachidonic acid metabolites that were released were identified by reverse-phase high-performance liquid chromatography. Leukotriene B4 and 5-hydroxyeicosatetraenoic acid were consistently observed. The lipoxygenase inhibitor, nordihydroguaiaretic acid, blocked production of these metabolites. The cyclooxygenase products, prostaglandin F2 alpha and thromboxane B2, were observed infrequently in comparison with leukotriene B4 and 5-hydroxyeicosatetraenoic acid.

Original languageEnglish (US)
Pages (from-to)1214-1217
Number of pages4
JournalAmerican journal of veterinary research
Volume49
Issue number8
StatePublished - Aug 1988
Externally publishedYes

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