Artificial polypeptide scaffold for protein immobilization

Kechun Zhang; Michael R. Diehl; David A. Tirrell

doi:10.1021/ja051457h

Artificial polypeptide scaffold for protein immobilization

Kechun Zhang, Michael R. Diehl, David A. Tirrell

Chemical Engineering and Materials Science

Research output: Contribution to journal › Article › peer-review

131 Scopus citations

Abstract

An artificial polypeptide scaffold composed of surface anchor and protein capture domains was designed and expressed in vivo. By using a mutant E. coli phenylalanyl-tRNA synthetase, the photoreactive amino acid para-azidophenylalanine was incorporated into the surface anchor domain. Octyltrichlorosilane-treated surfaces were functionalized with this polypeptide by spin coating and photocrosslinking. The resulting protein films were shown to immobilize recombinant proteins through association of coiled coil heterodimer.

Original language	English (US)
Pages (from-to)	10136-10137
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	127
Issue number	29
DOIs	https://doi.org/10.1021/ja051457h
State	Published - Jul 27 2005

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Artificial polypeptide scaffold for protein immobilization

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