Artificial polypeptide scaffold for protein immobilization

Kechun Zhang, Michael R. Diehl, David A. Tirrell

Research output: Contribution to journalArticlepeer-review

123 Scopus citations

Abstract

An artificial polypeptide scaffold composed of surface anchor and protein capture domains was designed and expressed in vivo. By using a mutant E. coli phenylalanyl-tRNA synthetase, the photoreactive amino acid para-azidophenylalanine was incorporated into the surface anchor domain. Octyltrichlorosilane-treated surfaces were functionalized with this polypeptide by spin coating and photocrosslinking. The resulting protein films were shown to immobilize recombinant proteins through association of coiled coil heterodimer.

Original languageEnglish (US)
Pages (from-to)10136-10137
Number of pages2
JournalJournal of the American Chemical Society
Volume127
Issue number29
DOIs
StatePublished - Jul 27 2005

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