TY - JOUR
T1 - Assays of thymic selection. Fetal thymus organ culture and in vitro thymocyte dulling assay.
AU - Hogquist, Kristin A
PY - 2001/2/12
Y1 - 2001/2/12
N2 - The in vitro methods of FTOC and DP dulling assay have been useful for defining the structural ligand requirements for positive selection of thymocytes. Both techniques, but especially the latter, have restrictions on their interpretation, which are outlined in the notes. The ability to study a cellular process in vitro has been, and will continue to be, of critical importance for immunologists. However, one should always be careful to confirm a hypothesis using in vivo techniques, whenever possible.
AB - The in vitro methods of FTOC and DP dulling assay have been useful for defining the structural ligand requirements for positive selection of thymocytes. Both techniques, but especially the latter, have restrictions on their interpretation, which are outlined in the notes. The ability to study a cellular process in vitro has been, and will continue to be, of critical importance for immunologists. However, one should always be careful to confirm a hypothesis using in vivo techniques, whenever possible.
UR - http://www.scopus.com/inward/record.url?scp=0035227070&partnerID=8YFLogxK
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M3 - Article
C2 - 11068763
AN - SCOPUS:0035227070
SN - 1064-3745
VL - 156
SP - 219
EP - 232
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -