TY - JOUR
T1 - Assessment of CD10 in the diagnosis of small B-cell lymphomas
T2 - A multiparameter flow cytometric study
AU - Xu, Yin
AU - McKenna, Robert W.
AU - Kroft, Steven H.
PY - 2002
Y1 - 2002
N2 - We evaluated the usefulness of multiparameter flow cytometry with cluster analysis in the diagnosis of a series of 100 well-characterized small B-cell lymphomas (SBCLs). The histologic diagnoses in the 100 cases were follicular lymphoma (FL) in 58, marginal zone lymphoma (MZL) in 17, small lymphocytic lymphoma in 15, and mantle cell lymphoma (MCL) in 10. Of the 58 FLs, 57 were CD10 positive (98% sensitivity). The 1 negative case was unusual in that it occurred in the small intestine. However, architectural, cytologic, and immunohistochemical features were diagnostic of FL. Of 42 other SBCLs, 2 were CDIO+ (95% specificity); 1 was a CD5+/cyclin D1 + MCL, and the other was an extranodal MZL. We found that assessment of CD10 expression using multiparameter flow cytometry with cluster analysis is highly sensitive and specific for the diagnosis of FL, validating its usefulness in situations in which adequate tissue is not available for definitive histologic diagnosis.
AB - We evaluated the usefulness of multiparameter flow cytometry with cluster analysis in the diagnosis of a series of 100 well-characterized small B-cell lymphomas (SBCLs). The histologic diagnoses in the 100 cases were follicular lymphoma (FL) in 58, marginal zone lymphoma (MZL) in 17, small lymphocytic lymphoma in 15, and mantle cell lymphoma (MCL) in 10. Of the 58 FLs, 57 were CD10 positive (98% sensitivity). The 1 negative case was unusual in that it occurred in the small intestine. However, architectural, cytologic, and immunohistochemical features were diagnostic of FL. Of 42 other SBCLs, 2 were CDIO+ (95% specificity); 1 was a CD5+/cyclin D1 + MCL, and the other was an extranodal MZL. We found that assessment of CD10 expression using multiparameter flow cytometry with cluster analysis is highly sensitive and specific for the diagnosis of FL, validating its usefulness in situations in which adequate tissue is not available for definitive histologic diagnosis.
KW - CD10
KW - Flow cytometry
KW - Immunophenotyping
KW - Lymphoma
KW - Small B-cell lymphoma
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U2 - 10.1309/T88X-71U4-WC0R-2531
DO - 10.1309/T88X-71U4-WC0R-2531
M3 - Article
C2 - 11863226
AN - SCOPUS:0036320073
SN - 0002-9173
VL - 117
SP - 291
EP - 300
JO - American journal of clinical pathology
JF - American journal of clinical pathology
IS - 2
ER -
