TY - GEN
T1 - Assessment of chlorinated ethenes biodegradation in an anaerobic aquifer by isotope analysis and microcosm studies
AU - Ebert, Karin
AU - Laskov, Christine
AU - Behrens, Sebastian
AU - Haderlein, Stefan
PY - 2011/12/1
Y1 - 2011/12/1
N2 - This work focused on identification and quantification of natural attenuation of chlorinated ethenes in a contaminated aquifer beneath an industrial area using compound specific isotope analysis (CSIA). Presence of cis-l,2-dichloroethene (cis-DCE) and vinylchloride (VC), as well as in situ redox conditions indicated degradation of the primary contaminant trichloroethene (TCE) by reductive dechlorination. The potential for VC degradation was further corroborated by PCR-analysis on water samples which confirmed the presence of Dehalococcoides strains and VC reductases. In situ biodegradation was estimated by various approaches, including concentration measurements along the groundwater flow path, microcosm studies and by compound specific stable carbon isotope analysis. Using the Rayleigh model carbon isotope enrichment factors, ε, were determined both in the field and in microcosm experiments for each dechlorination step. Estimates of biodegradation based on enrichment factors derived from microcosms (-8.6‰ (cis-DCE) and -27.2‰ (VC)) were consistently lower (up to 40%) than those based on field data. Our results of the isotope study at field scale, microcosm experiments and molecular marker analysis provided conclusive information on natural attenuation processes and can be recommended as a general approach for site characterisation and risk assessment in NA studies.
AB - This work focused on identification and quantification of natural attenuation of chlorinated ethenes in a contaminated aquifer beneath an industrial area using compound specific isotope analysis (CSIA). Presence of cis-l,2-dichloroethene (cis-DCE) and vinylchloride (VC), as well as in situ redox conditions indicated degradation of the primary contaminant trichloroethene (TCE) by reductive dechlorination. The potential for VC degradation was further corroborated by PCR-analysis on water samples which confirmed the presence of Dehalococcoides strains and VC reductases. In situ biodegradation was estimated by various approaches, including concentration measurements along the groundwater flow path, microcosm studies and by compound specific stable carbon isotope analysis. Using the Rayleigh model carbon isotope enrichment factors, ε, were determined both in the field and in microcosm experiments for each dechlorination step. Estimates of biodegradation based on enrichment factors derived from microcosms (-8.6‰ (cis-DCE) and -27.2‰ (VC)) were consistently lower (up to 40%) than those based on field data. Our results of the isotope study at field scale, microcosm experiments and molecular marker analysis provided conclusive information on natural attenuation processes and can be recommended as a general approach for site characterisation and risk assessment in NA studies.
KW - CSIA
KW - Carbon isotope fractionation
KW - Chlorinated ethenes
KW - Microcosm experiments
KW - Natural attenuation
UR - http://www.scopus.com/inward/record.url?scp=84860549179&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84860549179&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84860549179
SN - 9781907161162
T3 - IAHS-AISH Publication
SP - 231
EP - 234
BT - GQ10
T2 - 7th International Groundwater Quality Conference: Groundwater Quality Management in a Rapidly Changing World, GQ10
Y2 - 13 June 2010 through 18 June 2010
ER -