Three genes, SRY, ANT3, and CSF2RA, were mapped to the bovine Y chromosome (BTAY) by fluorescence in situ hybridization (FISH) and/or radiation hybrid (RH) mapping. FISH analysis indicated that the bovine SRY gene maps to the distal region of BTAYq, while ANT3 and CSF2RA are located in the pseudoautosomal region (PAR) of BTAYp and BTAXq. RH mapping with a 7000-rad cattle hamster whole-genome radiation hybrid panel further defined the ANT3 and CSF2RA position in relationship to previously mapped 12 PAR markers, and resulted in a relatively high resolution RH map for the PAR of BTAY.
Bibliographical noteFunding Information:
We thank Dr. Craig W. Beattie for providing the 7000-rad cattle hamster WGRH panel, Mr. David Galitz and Ms. Catherine Panke for technical assistance, and Mr. Benjamin Roelofs for helping with the CarthaGene software. This work was supported by a grant from USDA, NRICGP (2002-35205-11627) to F. A. Ponce de León and W. S. Liu.