Association of β1 integrin with protein kinase activity in large detergent resistant complexes

Amy P Skubitz; Kenneth D. Campbell; Said Goueli; Keith M Skubitz

doi:10.1016/S0014-5793(98)00380-9

Association of β1 integrin with protein kinase activity in large detergent resistant complexes

Amy P Skubitz, Kenneth D. Campbell, Said Goueli, Keith M Skubitz

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Integrins play a critical role in cell adhesion and mediate cell signaling. This report identifies the association of serine protein kinase activity with the β1 integrin by immunoprecipitation and phosphoamino acid analysis techniques. Reprecipitation techniques suggested that the serine kinase activity was not a member of the protein kinase C family. By gel filtration, most of the protein kinase activity associated with β1 integrin as well as most of the cell-surface β1 integrin was present in large detergent resistant complexes. These results suggest that serine protein kinase activity associated with the β1 integrin may play a role in signaling via the β1 integrin.

Original language	English (US)
Pages (from-to)	386-391
Number of pages	6
Journal	FEBS Letters
Volume	426
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(98)00380-9
State	Published - Apr 24 1998

Bibliographical note

Funding Information:
We would like to thank Drs. L. Furcht and A. Sonnenberg for providing antibodies and Dr. R. Bast, Jr. for providing cell lines and mAbs. Supported in part by NIH/NCI grant CA60658, the American Heart Association, Minnesota Affiliate, the Office of the Vice President for Research and Dean of the Graduate School of the University of Minnesota, the Minnesota Medical Foundation, and the Masonic Memorial Hospital Fund, Inc.

Keywords

Detergent resistant complex
Ovarian carcinoma
Protein kinase activity
β1 integrin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{f5a88c46d0384e5d9979affd9f3c554e,

title = "Association of β1 integrin with protein kinase activity in large detergent resistant complexes",

abstract = "Integrins play a critical role in cell adhesion and mediate cell signaling. This report identifies the association of serine protein kinase activity with the β1 integrin by immunoprecipitation and phosphoamino acid analysis techniques. Reprecipitation techniques suggested that the serine kinase activity was not a member of the protein kinase C family. By gel filtration, most of the protein kinase activity associated with β1 integrin as well as most of the cell-surface β1 integrin was present in large detergent resistant complexes. These results suggest that serine protein kinase activity associated with the β1 integrin may play a role in signaling via the β1 integrin.",

keywords = "Detergent resistant complex, Ovarian carcinoma, Protein kinase activity, β1 integrin",

author = "Skubitz, {Amy P} and Campbell, {Kenneth D.} and Said Goueli and Skubitz, {Keith M}",

note = "Funding Information: We would like to thank Drs. L. Furcht and A. Sonnenberg for providing antibodies and Dr. R. Bast, Jr. for providing cell lines and mAbs. Supported in part by NIH/NCI grant CA60658, the American Heart Association, Minnesota Affiliate, the Office of the Vice President for Research and Dean of the Graduate School of the University of Minnesota, the Minnesota Medical Foundation, and the Masonic Memorial Hospital Fund, Inc.",

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doi = "10.1016/S0014-5793(98)00380-9",

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T1 - Association of β1 integrin with protein kinase activity in large detergent resistant complexes

AU - Skubitz, Amy P

AU - Campbell, Kenneth D.

AU - Goueli, Said

AU - Skubitz, Keith M

N1 - Funding Information: We would like to thank Drs. L. Furcht and A. Sonnenberg for providing antibodies and Dr. R. Bast, Jr. for providing cell lines and mAbs. Supported in part by NIH/NCI grant CA60658, the American Heart Association, Minnesota Affiliate, the Office of the Vice President for Research and Dean of the Graduate School of the University of Minnesota, the Minnesota Medical Foundation, and the Masonic Memorial Hospital Fund, Inc.

PY - 1998/4/24

Y1 - 1998/4/24

N2 - Integrins play a critical role in cell adhesion and mediate cell signaling. This report identifies the association of serine protein kinase activity with the β1 integrin by immunoprecipitation and phosphoamino acid analysis techniques. Reprecipitation techniques suggested that the serine kinase activity was not a member of the protein kinase C family. By gel filtration, most of the protein kinase activity associated with β1 integrin as well as most of the cell-surface β1 integrin was present in large detergent resistant complexes. These results suggest that serine protein kinase activity associated with the β1 integrin may play a role in signaling via the β1 integrin.

AB - Integrins play a critical role in cell adhesion and mediate cell signaling. This report identifies the association of serine protein kinase activity with the β1 integrin by immunoprecipitation and phosphoamino acid analysis techniques. Reprecipitation techniques suggested that the serine kinase activity was not a member of the protein kinase C family. By gel filtration, most of the protein kinase activity associated with β1 integrin as well as most of the cell-surface β1 integrin was present in large detergent resistant complexes. These results suggest that serine protein kinase activity associated with the β1 integrin may play a role in signaling via the β1 integrin.

KW - Detergent resistant complex

KW - Ovarian carcinoma

KW - Protein kinase activity

KW - β1 integrin

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SN - 0014-5793

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EP - 391

JO - FEBS Letters

JF - FEBS Letters

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Association of β1 integrin with protein kinase activity in large detergent resistant complexes

Abstract

Bibliographical note

Keywords

UN SDGs

Access

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