Atrial natriuretic factor inhibits norepinephrine release in an adrenergic clonal cell line (PC12)

James Drewett; Gary Marchand; Richard Ziegler; George J Trachte

doi:10.1016/0014-2999(88)90765-0

Atrial natriuretic factor inhibits norepinephrine release in an adrenergic clonal cell line (PC12)

James Drewett, Gary Marchand, Richard Ziegler, George J Trachte

Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Evidence for a presynaptic neuromodulatory effect of atrial natriuretic factor (ANF) has been obtained in rat adrenal pheochromocytoma cells (PC12), a clonal line that differentiates into adrenergic neuron-like cells when treated with nerve growth factor. ANF had no effect on basal norepinephrine (NE) release. In contrast, ANF markedly and significantly inhibited carbachol-induced NE release in a concentration-dependent manner. This result is consistent with the hypothesis that ANF may be an inhibitory neuromodulator.

Original language	English (US)
Pages (from-to)	175-179
Number of pages	5
Journal	European Journal of Pharmacology
Volume	150
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-2999(88)90765-0
State	Published - May 20 1988

Bibliographical note

Funding Information:
The expert technical assistance of Mary Sneve and the expert clerical assistance of Susan Kurki is greatly appreciated. We also thank Dr. B. Wigdahl for supplying the PC12 cells. This work was supported by the Public Heatlh Service Grants RO1 HL35934 and RO1 HL34600 and by Biomedical Research Support Grant SO7 RR05896.

Keywords

(Cell-line)
Atrial natriuretic factor (ANF)
Norepinephrine
Pheochromocytoma cells (PC12)
Sympathetic nervous system

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title = "Atrial natriuretic factor inhibits norepinephrine release in an adrenergic clonal cell line (PC12)",

abstract = "Evidence for a presynaptic neuromodulatory effect of atrial natriuretic factor (ANF) has been obtained in rat adrenal pheochromocytoma cells (PC12), a clonal line that differentiates into adrenergic neuron-like cells when treated with nerve growth factor. ANF had no effect on basal norepinephrine (NE) release. In contrast, ANF markedly and significantly inhibited carbachol-induced NE release in a concentration-dependent manner. This result is consistent with the hypothesis that ANF may be an inhibitory neuromodulator.",

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note = "Funding Information: The expert technical assistance of Mary Sneve and the expert clerical assistance of Susan Kurki is greatly appreciated. We also thank Dr. B. Wigdahl for supplying the PC12 cells. This work was supported by the Public Heatlh Service Grants RO1 HL35934 and RO1 HL34600 and by Biomedical Research Support Grant SO7 RR05896.",

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AU - Ziegler, Richard

AU - Trachte, George J

N1 - Funding Information: The expert technical assistance of Mary Sneve and the expert clerical assistance of Susan Kurki is greatly appreciated. We also thank Dr. B. Wigdahl for supplying the PC12 cells. This work was supported by the Public Heatlh Service Grants RO1 HL35934 and RO1 HL34600 and by Biomedical Research Support Grant SO7 RR05896.

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N2 - Evidence for a presynaptic neuromodulatory effect of atrial natriuretic factor (ANF) has been obtained in rat adrenal pheochromocytoma cells (PC12), a clonal line that differentiates into adrenergic neuron-like cells when treated with nerve growth factor. ANF had no effect on basal norepinephrine (NE) release. In contrast, ANF markedly and significantly inhibited carbachol-induced NE release in a concentration-dependent manner. This result is consistent with the hypothesis that ANF may be an inhibitory neuromodulator.

AB - Evidence for a presynaptic neuromodulatory effect of atrial natriuretic factor (ANF) has been obtained in rat adrenal pheochromocytoma cells (PC12), a clonal line that differentiates into adrenergic neuron-like cells when treated with nerve growth factor. ANF had no effect on basal norepinephrine (NE) release. In contrast, ANF markedly and significantly inhibited carbachol-induced NE release in a concentration-dependent manner. This result is consistent with the hypothesis that ANF may be an inhibitory neuromodulator.

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KW - Sympathetic nervous system

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Atrial natriuretic factor inhibits norepinephrine release in an adrenergic clonal cell line (PC12)

Abstract

Bibliographical note

Keywords

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