Automated continuous flow Polymerase Chain Reaction in independent microdroplets

Max Chabert, Kevin D. Dorfman, Jean Louis Viovy

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a fully automated system for performing continuous sampling, reagent mixing and Polymerase Chain Reaction (PCR) in microdroplets transported in immiscible oil. Reproducible amplification, negligible cross contamination and low detection limits were demonstrated in continuous operation on numerous consecutive sample drops. The system, which opens the route to strong reagents and labor savings in high throughput applications, was validated on the clinically relevant quantification of progesterone receptor gene expression in human breast cancer cell lines.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference
Subtitle of host publication10th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherJapan Academic Association Inc
Pages1220-1222
Number of pages3
StatePublished - Jan 1 2006
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: Nov 5 2006Nov 9 2006

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period11/5/0611/9/06

Keywords

  • Automation
  • Continuous flow
  • Droplets
  • Interface
  • PCR

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