AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis cullin AtCUL1 is required for auxin response

Juan C. Del Pozo; Sunethra Dharmasiri; Hanjo Hellmann; Loni Walker; William M. Gray; Mark Estelle

doi:10.1105/tpc.010282

AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis cullin AtCUL1 is required for auxin response

Juan C. Del Pozo, Sunethra Dharmasiri, Hanjo Hellmann, Loni Walker, William M. Gray, Mark Estelle

Plant and Microbial Biology

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Abstract

Mutations in the AXR1 gene result in a reduction in auxin response and diverse defects in auxin-regulated growth and development. In a previous study, we showed that AXR1 forms a heteradimer with the ECR1 protein. This enzyme activates the ubiquitin-related protein RUB1 in vitro. Furthermore, we showed that the Skp1-Cul1/Cdc53-F-bax (SCF) subunit AtCUL1 is modified by RUB1 in viva. In this report, we demonstrate that the formation of RUB-AtCUL1 is dependent on AXR1 and ECR1 in viva. The expression of AXR1 and ECR1 is restricted to zones of active cell division and cell elongation, consistent with their role in growth regulation. These results provide strong support for a model in which RUB conjugation of AtCUL1 affects the function of SCF E3s that are required for auxin response.

Original language	English (US)
Pages (from-to)	421-433
Number of pages	13
Journal	Plant Cell
Volume	14
Issue number	2
DOIs	https://doi.org/10.1105/tpc.010282
State	Published - 2002

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title = "AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis cullin AtCUL1 is required for auxin response",

abstract = "Mutations in the AXR1 gene result in a reduction in auxin response and diverse defects in auxin-regulated growth and development. In a previous study, we showed that AXR1 forms a heteradimer with the ECR1 protein. This enzyme activates the ubiquitin-related protein RUB1 in vitro. Furthermore, we showed that the Skp1-Cul1/Cdc53-F-bax (SCF) subunit AtCUL1 is modified by RUB1 in viva. In this report, we demonstrate that the formation of RUB-AtCUL1 is dependent on AXR1 and ECR1 in viva. The expression of AXR1 and ECR1 is restricted to zones of active cell division and cell elongation, consistent with their role in growth regulation. These results provide strong support for a model in which RUB conjugation of AtCUL1 affects the function of SCF E3s that are required for auxin response.",

author = "{Del Pozo}, {Juan C.} and Sunethra Dharmasiri and Hanjo Hellmann and Loni Walker and Gray, {William M.} and Mark Estelle",

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T1 - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis cullin AtCUL1 is required for auxin response

AU - Del Pozo, Juan C.

AU - Dharmasiri, Sunethra

AU - Hellmann, Hanjo

AU - Walker, Loni

AU - Gray, William M.

AU - Estelle, Mark

PY - 2002

Y1 - 2002

N2 - Mutations in the AXR1 gene result in a reduction in auxin response and diverse defects in auxin-regulated growth and development. In a previous study, we showed that AXR1 forms a heteradimer with the ECR1 protein. This enzyme activates the ubiquitin-related protein RUB1 in vitro. Furthermore, we showed that the Skp1-Cul1/Cdc53-F-bax (SCF) subunit AtCUL1 is modified by RUB1 in viva. In this report, we demonstrate that the formation of RUB-AtCUL1 is dependent on AXR1 and ECR1 in viva. The expression of AXR1 and ECR1 is restricted to zones of active cell division and cell elongation, consistent with their role in growth regulation. These results provide strong support for a model in which RUB conjugation of AtCUL1 affects the function of SCF E3s that are required for auxin response.

AB - Mutations in the AXR1 gene result in a reduction in auxin response and diverse defects in auxin-regulated growth and development. In a previous study, we showed that AXR1 forms a heteradimer with the ECR1 protein. This enzyme activates the ubiquitin-related protein RUB1 in vitro. Furthermore, we showed that the Skp1-Cul1/Cdc53-F-bax (SCF) subunit AtCUL1 is modified by RUB1 in viva. In this report, we demonstrate that the formation of RUB-AtCUL1 is dependent on AXR1 and ECR1 in viva. The expression of AXR1 and ECR1 is restricted to zones of active cell division and cell elongation, consistent with their role in growth regulation. These results provide strong support for a model in which RUB conjugation of AtCUL1 affects the function of SCF E3s that are required for auxin response.

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AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis cullin AtCUL1 is required for auxin response

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