Azidoatrazine: Photoaffinity label for the site of triazine herbicide action in chloroplasts

Gary Gardner

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

Binding of the 4-azido analog of the herbicide atrazine to pea chloroplast membranes was compared with that of atrazine. When [14C]azidoatrazine was treated with 300-nanometer ultraviolet light in situ, reversibility of binding was lost in proportion to the duration of irradiation. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of chloroplast membranes irradiated in the presence of [14C]azidoatrazine indicated radioactivity in only one region, corresponding to a protein with a molecular weight of approximately 32,000. Azidoatrazine is a photoaffinity reagent for the triazine binding site in chloroplasts and serves as a label to identify this site, which may be the apoprotein of the secondary electron acceptor in photosystem II.

Original languageEnglish (US)
Pages (from-to)937-940
Number of pages4
JournalScience
Volume211
Issue number4485
DOIs
StatePublished - Jan 1 1981

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