B and T cell epitope mapping and study the humoral and cell mediated immune response to B-T constructs of YscF antigen of Yersinia pestis

Riyasat Ali, Sudhir Kumar, Raza Ali Naqvi, D. N. Rao

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

YscF antigen, a type III secretion protein has recently been shown partial protection in murine model. Five peptides of YscF antigen were predicted using DNASTAR and T-cell prediction software. Peptides were synthesised and authenticated using competitive, direct binding immunoassay with anti YscF/peptide sera raised in mice. Peptide P1 and P2 were found to be B cell epitope while P3 was minor B cell epitope. P4 peptide was a pure T cell epitope based on lymphoproliferative response, cytokines profile and T-bet expression. Furthermore, with an intention to enhance immunogenicity, three B-T constructs were designed between the above epitopes. Conjugate B1T1 and B2T1 showed higher serum IgG/IgA titre, respectively, as well as high secretory IgA plus secretory component (Sc) both in lung and intestinal washes. Also, these conjugates showed high T-cell proliferation in addition to higher Th1 type cytokines (IFN-γ and IL-2) in cells obtained from spleen, lamina propria and Peyer's patches. B3T1 stimulated cells showed moderate levels of IFN-γ and IL-2 but higher levels of IL-4. This study demonstrates superior immunogen of B1T1 and B2T1 of YscF antigen to be exploited as vaccine candidate for plague.

Original languageEnglish (US)
Pages (from-to)365-378
Number of pages14
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume36
Issue number4
DOIs
StatePublished - Jul 2013

Bibliographical note

Funding Information:
The authors are grateful to the Indian Council of Medical Research, New Delhi for financial assistance to carry out the study and Council for Scientific and Industrial Research to provide fellowship to Mr. Riyasat Ali. Special thanks to Dr. Wieslaw Swietnicki (USAMRIID) and Dr. Jyl Matson from USA for kind gift of recombinant YscF antigen. Appendix A

Keywords

  • B-T construct
  • Cytokine
  • Epitope
  • Yersinia pestis
  • YscF antigen

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