B-cell lymphoproliferative disorders in solid-organ transplant patients: Detection of Epstein-Barr virus by in situ hybridization

Laurence C. Berg; Cedith M. Copenhaver; Vicki A. Morrison; Scott A. Gruber; David L. Dunn; K. Gajl-Peczalska; John G. Strickler

doi:10.1016/0046-8177(92)90237-W

B-cell lymphoproliferative disorders in solid-organ transplant patients: Detection of Epstein-Barr virus by in situ hybridization

Laurence C. Berg, Cedith M. Copenhaver, Vicki A. Morrison, Scott A. Gruber, David L. Dunn, K. Gajl-Peczalska, John G. Strickler

Medicine - Hematology, Oncology, Transplant

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35 Scopus citations

Abstract

B-cell lymphoproliferative disorders (BLPDs) occur in approximately 2% of transplant recipients and are frequently fatal. Indirect serologic evidence has implicated Epstein-Barr virus (EBV) as an etiologic factor in these lesions. Direct evidence of the presence of EBV in these lesions has been obtained in relatively few cases. We used in situ hybridization (ISH) with a probe for the BamHI-W region of the EBV genome to study 52 tissue specimens from 28 solidorgan transplant patients who had BLPD. Epstein-Barr virus-infected lymphoid cells were identified in 26 of these 28 patients. The two patients without ISH evidence of EBV infection showed no distinctive clinical, morphologic, or serologic features. Previous filter-hybridization studies of these two patients had demonstrated evidence of EBV infection. Seven additional transplant patients without evidence of BLPD were studied as controls and showed no evidence of EBV in their lymphoid cells by ISH. These data provide further support for the etiologic role of EBV in the pathogenesis of posttransplantation lymphoproliferative disorders.

Original language	English (US)
Pages (from-to)	159-163
Number of pages	5
Journal	Human pathology
Volume	23
Issue number	2
DOIs	https://doi.org/10.1016/0046-8177(92)90237-W
State	Published - Feb 1992

Bibliographical note

Funding Information:
Fn,nr thr Drpwtments of Pathology. Medicine, and Surgery. liniversit) of Minnesota, Minneapolis, MN: and the Department of P,~thology, Wilford Hal1 Medical Center. Lackland Air Force Base. TN. Accepted for publication April 24, 199 1. Supported in part by a grant from the Minnesota Medical Foundation (J. G. 5%).n -search grant AM 13083 from the National Institutes of Health, and a grant from Sandoz Pharmaceuticals (Lxporation (5. A. G.). &T uro,dx Epstein-Barr virus, in situ hybridization, posttrans-plantation lymphoproliferative disorder. ‘The views expressed in this article are those of the authors and do not reflect the official policy of the Department of Defense or other departments of the LJS government. .Addres!, correspondence and reprint requests to John (;. Strickler. MD. Section of Surgical Pathology. Mayo Clinic and Mayo Foundation. Rochester. MN 5590.5. Copyright 6 1W ? by W.B. Saunders Compmy 0046-81 77/O”,CIO?-00 11$5.00/0

Keywords

Epstein-Barr virus
in situ hybridization
posttransplantation lymphoproliferative disorder

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@article{0b38cb5e4a6e4bab8dd4200956e1eba3,

title = "B-cell lymphoproliferative disorders in solid-organ transplant patients: Detection of Epstein-Barr virus by in situ hybridization",

abstract = "B-cell lymphoproliferative disorders (BLPDs) occur in approximately 2% of transplant recipients and are frequently fatal. Indirect serologic evidence has implicated Epstein-Barr virus (EBV) as an etiologic factor in these lesions. Direct evidence of the presence of EBV in these lesions has been obtained in relatively few cases. We used in situ hybridization (ISH) with a probe for the BamHI-W region of the EBV genome to study 52 tissue specimens from 28 solidorgan transplant patients who had BLPD. Epstein-Barr virus-infected lymphoid cells were identified in 26 of these 28 patients. The two patients without ISH evidence of EBV infection showed no distinctive clinical, morphologic, or serologic features. Previous filter-hybridization studies of these two patients had demonstrated evidence of EBV infection. Seven additional transplant patients without evidence of BLPD were studied as controls and showed no evidence of EBV in their lymphoid cells by ISH. These data provide further support for the etiologic role of EBV in the pathogenesis of posttransplantation lymphoproliferative disorders.",

keywords = "Epstein-Barr virus, in situ hybridization, posttransplantation lymphoproliferative disorder",

author = "Berg, {Laurence C.} and Copenhaver, {Cedith M.} and Morrison, {Vicki A.} and Gruber, {Scott A.} and Dunn, {David L.} and K. Gajl-Peczalska and Strickler, {John G.}",

note = "Funding Information: Fn,nr thr Drpwtments of Pathology. Medicine, and Surgery. liniversit) of Minnesota, Minneapolis, MN: and the Department of P,~thology, Wilford Hal1 Medical Center. Lackland Air Force Base. TN. Accepted for publication April 24, 199 1. Supported in part by a grant from the Minnesota Medical Foundation (J. G. 5%).n -search grant AM 13083 from the National Institutes of Health, and a grant from Sandoz Pharmaceuticals (Lxporation (5. A. G.). &T uro,dx Epstein-Barr virus, in situ hybridization, posttrans-plantation lymphoproliferative disorder. {\textquoteleft}The views expressed in this article are those of the authors and do not reflect the official policy of the Department of Defense or other departments of the LJS government. .Addres!, correspondence and reprint requests to John (;. Strickler. MD. Section of Surgical Pathology. Mayo Clinic and Mayo Foundation. Rochester. MN 5590.5. Copyright 6 1W ? by W.B. Saunders Compmy 0046-81 77/O”,CIO?-00 11$5.00/0",

year = "1992",

month = feb,

doi = "10.1016/0046-8177(92)90237-W",

language = "English (US)",

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AU - Copenhaver, Cedith M.

AU - Morrison, Vicki A.

AU - Gruber, Scott A.

AU - Dunn, David L.

AU - Gajl-Peczalska, K.

AU - Strickler, John G.

N1 - Funding Information: Fn,nr thr Drpwtments of Pathology. Medicine, and Surgery. liniversit) of Minnesota, Minneapolis, MN: and the Department of P,~thology, Wilford Hal1 Medical Center. Lackland Air Force Base. TN. Accepted for publication April 24, 199 1. Supported in part by a grant from the Minnesota Medical Foundation (J. G. 5%).n -search grant AM 13083 from the National Institutes of Health, and a grant from Sandoz Pharmaceuticals (Lxporation (5. A. G.). &T uro,dx Epstein-Barr virus, in situ hybridization, posttrans-plantation lymphoproliferative disorder. ‘The views expressed in this article are those of the authors and do not reflect the official policy of the Department of Defense or other departments of the LJS government. .Addres!, correspondence and reprint requests to John (;. Strickler. MD. Section of Surgical Pathology. Mayo Clinic and Mayo Foundation. Rochester. MN 5590.5. Copyright 6 1W ? by W.B. Saunders Compmy 0046-81 77/O”,CIO?-00 11$5.00/0

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N2 - B-cell lymphoproliferative disorders (BLPDs) occur in approximately 2% of transplant recipients and are frequently fatal. Indirect serologic evidence has implicated Epstein-Barr virus (EBV) as an etiologic factor in these lesions. Direct evidence of the presence of EBV in these lesions has been obtained in relatively few cases. We used in situ hybridization (ISH) with a probe for the BamHI-W region of the EBV genome to study 52 tissue specimens from 28 solidorgan transplant patients who had BLPD. Epstein-Barr virus-infected lymphoid cells were identified in 26 of these 28 patients. The two patients without ISH evidence of EBV infection showed no distinctive clinical, morphologic, or serologic features. Previous filter-hybridization studies of these two patients had demonstrated evidence of EBV infection. Seven additional transplant patients without evidence of BLPD were studied as controls and showed no evidence of EBV in their lymphoid cells by ISH. These data provide further support for the etiologic role of EBV in the pathogenesis of posttransplantation lymphoproliferative disorders.

AB - B-cell lymphoproliferative disorders (BLPDs) occur in approximately 2% of transplant recipients and are frequently fatal. Indirect serologic evidence has implicated Epstein-Barr virus (EBV) as an etiologic factor in these lesions. Direct evidence of the presence of EBV in these lesions has been obtained in relatively few cases. We used in situ hybridization (ISH) with a probe for the BamHI-W region of the EBV genome to study 52 tissue specimens from 28 solidorgan transplant patients who had BLPD. Epstein-Barr virus-infected lymphoid cells were identified in 26 of these 28 patients. The two patients without ISH evidence of EBV infection showed no distinctive clinical, morphologic, or serologic features. Previous filter-hybridization studies of these two patients had demonstrated evidence of EBV infection. Seven additional transplant patients without evidence of BLPD were studied as controls and showed no evidence of EBV in their lymphoid cells by ISH. These data provide further support for the etiologic role of EBV in the pathogenesis of posttransplantation lymphoproliferative disorders.

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ER -

B-cell lymphoproliferative disorders in solid-organ transplant patients: Detection of Epstein-Barr virus by in situ hybridization

Abstract

Bibliographical note

Keywords

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