Abstract
The backbone dynamics of the λ-Cro represser protein have been studied by analyzing 15N relaxation rates and the nuclear Overhauser enhancement in terms of a model-free formalism. An efficient method has been developed for obtaining the local motional parameters from the observed data by the least-squares method in which only the overall correlation time can be effectively adjusted as a non-linear parameter. The calculated relaxation parameters of the Cro protein indicated that its C-terminal region corresponding to the DNA binding site is quite mobile.
Original language | English (US) |
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Pages (from-to) | 143-150 |
Number of pages | 8 |
Journal | Journal of Molecular Structure |
Volume | 379 |
Issue number | 1-3 |
DOIs | |
State | Published - Jan 1 1996 |
Keywords
- Backbone dynamics
- Cro represser
- Least squares method
- Model-free formalism
- N relaxation rate