Backbone dynamics of the λ-Cro represser protein determined by15N relaxation measurements. Application of an efficient method for calculation of dynamics parameters

Hiroshi Matsuo, Hiromu Sugeta, Masahiro Shirakawa, Yoshimasa Kyogoku

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The backbone dynamics of the λ-Cro represser protein have been studied by analyzing 15N relaxation rates and the nuclear Overhauser enhancement in terms of a model-free formalism. An efficient method has been developed for obtaining the local motional parameters from the observed data by the least-squares method in which only the overall correlation time can be effectively adjusted as a non-linear parameter. The calculated relaxation parameters of the Cro protein indicated that its C-terminal region corresponding to the DNA binding site is quite mobile.

Original languageEnglish (US)
Pages (from-to)143-150
Number of pages8
JournalJournal of Molecular Structure
Volume379
Issue number1-3
DOIs
StatePublished - Jan 1 1996

Keywords

  • Backbone dynamics
  • Cro represser
  • Least squares method
  • Model-free formalism
  • N relaxation rate

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