Backbone NMR resonance assignment of the catalytic subunit of cAMP-dependent protein kinase A in complex with AMP-PNP

Larry R. Masterson; Lei Shi; Marco Tonelli; Alessandro Mascioni; Michael M. Mueller; Gianluigi Veglia

doi:10.1007/s12104-009-9154-8

Backbone NMR resonance assignment of the catalytic subunit of cAMP-dependent protein kinase A in complex with AMP-PNP

Larry R. Masterson, Lei Shi, Marco Tonelli, Alessandro Mascioni, Michael M. Mueller, Gianluigi Veglia

Chemical and Structural Biology (TMED)

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The catalytic subunit of protein kinase A is involved with a number of signal transduction pathways and has been used as a benchmark to study the structural biology and biochemistry for the entire kinase family of enzymes. Here, we report the backbone assignment of the intact 41 kDa catalytic subunit bound to AMP-PNP.

Original language	English (US)
Pages (from-to)	115-117
Number of pages	3
Journal	Biomolecular NMR Assignments
Volume	3
Issue number	1
DOIs	https://doi.org/10.1007/s12104-009-9154-8
State	Published - Jun 2009

Bibliographical note

Funding Information:
Acknowledgments This work was supported by NIH grants GM64742 and HL080081 (G.V.), and GM08700 (L.R.M.). The University of Minnesota NMR facility is supported by NSF funding BIR-961477 and the University of Minnesota Medical Foundation. NMRFAM is supported by NIH grants P41RR02301, P41GM66326, RR02781, and RR08438, along with NSF grants DMB-8415048, OIA-9977486, and BIR-9214394.

Keywords

CCLS-HSQC
Dual amino acid selective labeling
NMR
Protein backbone resonance assignments
Protein kinase A

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abstract = "The catalytic subunit of protein kinase A is involved with a number of signal transduction pathways and has been used as a benchmark to study the structural biology and biochemistry for the entire kinase family of enzymes. Here, we report the backbone assignment of the intact 41 kDa catalytic subunit bound to AMP-PNP.",

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AU - Mascioni, Alessandro

AU - Mueller, Michael M.

AU - Veglia, Gianluigi

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Backbone NMR resonance assignment of the catalytic subunit of cAMP-dependent protein kinase A in complex with AMP-PNP

Abstract

Bibliographical note

Keywords

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