TY - JOUR
T1 - Bacterial translocation in cultured enterocytes
T2 - Magnitude, specificity, and electron microscopic observations of endocytosis
AU - Wells, Carol L
AU - Jechorek, Robert P.
AU - Olmsted, Stephen B.
AU - Erlandsen, Stanley L.
PY - 1994/6
Y1 - 1994/6
N2 - Previous in vivo evidence has shown that bacterial phagocytosis by enterocytes may be an initial step in bacterial translocation across the intestinal epithelium. This study analyzed the interactions of cultured enterocytes, namely Caco-2 cells, with nine strains of enteric bacteria, tested in pure culture and in mixed culture. These nine strains had a spectrum of invasive potential and included Salmonella typhimurium, Listeria monocytogenes (three strains), Escherichia coli (three strains), Proteus mirabilis, and Enterococcus faecalis. Numbers of viable intracellular bacteria recovered from Caco-2 cells were: L. monocytogenes>S. typhimurium>P. mirabilis>E. coli>E. faecalis. Uptake of a given microbe by enterocytes was strain-specific and was not influenced by the presence of another strain, regardless of the invasive ability of the coinfecting strain. Electron microscopic visualization of bacterial adherence and uptake by Caco-2 cells indicated that the epithelial interactions of normal enteric bacteria were similar to these observed with invasive strains of salmonella and listeria.
AB - Previous in vivo evidence has shown that bacterial phagocytosis by enterocytes may be an initial step in bacterial translocation across the intestinal epithelium. This study analyzed the interactions of cultured enterocytes, namely Caco-2 cells, with nine strains of enteric bacteria, tested in pure culture and in mixed culture. These nine strains had a spectrum of invasive potential and included Salmonella typhimurium, Listeria monocytogenes (three strains), Escherichia coli (three strains), Proteus mirabilis, and Enterococcus faecalis. Numbers of viable intracellular bacteria recovered from Caco-2 cells were: L. monocytogenes>S. typhimurium>P. mirabilis>E. coli>E. faecalis. Uptake of a given microbe by enterocytes was strain-specific and was not influenced by the presence of another strain, regardless of the invasive ability of the coinfecting strain. Electron microscopic visualization of bacterial adherence and uptake by Caco-2 cells indicated that the epithelial interactions of normal enteric bacteria were similar to these observed with invasive strains of salmonella and listeria.
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U2 - 10.1097/00024382-199406000-00009
DO - 10.1097/00024382-199406000-00009
M3 - Article
C2 - 7735974
AN - SCOPUS:0028458657
SN - 1073-2322
VL - 1
SP - 443
EP - 451
JO - Shock
JF - Shock
IS - 6
ER -