Bacterially preexposed T cells impair bacterial elimination by non- Th1/Th2 cell mechanisms in a model of intra-abdominal infection

T. G. Gleason, R. G. Sawyer, T. L. Pruett, P. E. Bankey, J. A. Lederer, J. Alverdy

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Background. Escherichia coli preexposure in mice results in impaired elimination of subsequent intra-abdominal infections by a CD4+ T cell- dependent process. Certain gram-negative infections have been shown to induce T-helper-(Th)2-type CD4+ T-cell differentiation, which correlates with impaired elimination of infection and death. We hypothesized that E coli preexposure impairs subsequent bacterial elimination as a consequence of Th2 differentiation and that interleukin-12 (IL-12) treatment could reverse this differentiation and minimize the effects of E coli preexposure. Methods. After preexposure to E coli or other species, BALB/c mice or interferon-T (IFN-γ)-deficient mice, treated with or without IL-12, were given a standard intra-abdominal infection (E coli, Bacteroides fragilis, and adjuvant). Cohorts were killed for abscess quantification, in vitro T-cell proliferative responsiveness, and cytokine secretory profiles. Splenic lymphocytes preexposed in vivo to other types of bacteria were transferred to naive mice before intra-abdominal infection to determine whether preexposure, eliciting the lymphocyte-dependent response, was species specific. Results. E coli preexposure alone caused no Th1 or Th2 shift; increased the proliferative responses of T cells; and, in combination with IL-12 therapy, caused markedly decreased IL-2 and IL-4 responses and an increased IFN-γ response. IL-12 therapy did not change the response to intra-abdominal infection despite its ability to cause marked Th1 polarization. IFN-γ-deficient mice responded to E coli preexposure no differently than did wild-type mice. Transfer of lymphocytes preexposed to Pseudomonas aeruginosa, Klebsiella pneumoniae, and hemolytic E coli but not other types of nosocomial pathogens caused the development of more abscesses just as transfer of E coli preexposed lymphocytes had. Conclusions. CD4+ T cells responsive to E coli preexposure regulate subsequent intra-abdominal abscess formation by a mechanism not explained by the Th1/Th2 paradigm. Preexposure to hemolytic E coli and other Enterobacteriaceae alters responses to intra-abdominal infection.

Original languageEnglish (US)
Pages (from-to)418-428
Number of pages11
JournalSurgery
Volume124
Issue number2
DOIs
StatePublished - 1998

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