BED domain-containing NLR from wild barley confers resistance to leaf rust

Chunhong Chen; Matthias Jost; Bethany Clark; Matthew Martin; Oadi Matny; Brian J. Steffenson; Jerome D. Franckowiak; Martin Mascher; Davinder Singh; Dragan Perovic; Terese Richardson; Sambasivam Periyannan; Evans S. Lagudah; Robert F. Park; Peter M. Dracatos

doi:10.1111/pbi.13542

BED domain-containing NLR from wild barley confers resistance to leaf rust

Chunhong Chen, Matthias Jost, Bethany Clark, Matthew Martin, Oadi Matny, Brian J. Steffenson, Jerome D. Franckowiak, Martin Mascher, Davinder Singh, Dragan Perovic, Terese Richardson, Sambasivam Periyannan, Evans S. Lagudah, Robert F. Park, Peter M. Dracatos

Plant Pathology

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Leaf rust, caused by Puccinia hordei, is a devastating fungal disease affecting barley (Hordeum vulgare subsp. vulgare) production globally. Despite the effectiveness of genetic resistance, the deployment of single genes often compromises durability due to the emergence of virulent P. hordei races, prompting the search for new sources of resistance. Here we report on the cloning of Rph15, a resistance gene derived from barley’s wild progenitor H. vulgare subsp. spontaneum. We demonstrate using introgression mapping, mutation and complementation that the Rph15 gene from the near-isogenic line (NIL) Bowman + Rph15 (referred to as BW719) encodes a coiled-coil nucleotide-binding leucine-rich repeat (NLR) protein with an integrated Zinc finger BED (ZF-BED) domain. A predicted KASP marker was developed and validated across a collection of Australian cultivars and a series of introgression lines in the Bowman background known to carry the Rph15 resistance. Rph16 from HS-680, another wild barley derived leaf rust resistance gene, was previously mapped to the same genomic region on chromosome 2H and was assumed to be allelic with Rph15 based on genetic studies. Both sequence analysis, race specificity and the identification of a knockout mutant in the HS-680 background suggest that Rph15- and Rph16-mediated resistances are in fact the same and not allelic as previously thought. The cloning of Rph15 now permits efficient gene deployment and the production of resistance gene cassettes for sustained leaf rust control.

Original language	English (US)
Pages (from-to)	1206-1215
Number of pages	10
Journal	Plant Biotechnology Journal
Volume	19
Issue number	6
DOIs	https://doi.org/10.1111/pbi.13542
State	Published - Jun 2021

Bibliographical note

Funding Information:
This work was supported by funds provided through Grains and Development Corporation (US00074), Judith and David Coffey and family and the Lieberman-Okinow Endowment at the University of Minnesota. We are grateful to Matthew Williams, Dorian Friendship (University of Sydney), Smitha Louis and Dhara Bhatt (CSIRO Agriculture and Food, Australia) for technical assistance. The authors also thank Dr. Peter Dodds and Dr. Michael Ayliffe for commenting on the manuscript.

Funding Information:
This work was supported by funds provided through Grains and Development Corporation (US00074), Judith and David Coffey and family and the Lieberman‐Okinow Endowment at the University of Minnesota. We are grateful to Matthew Williams, Dorian Friendship (University of Sydney), Smitha Louis and Dhara Bhatt (CSIRO Agriculture and Food, Australia) for technical assistance. The authors also thank Dr. Peter Dodds and Dr. Michael Ayliffe for commenting on the manuscript.

Publisher Copyright:
© 2021 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

Keywords

Gene cloning
NLR
leaf rust resistance
wild barley

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access

10.1111/pbi.13542

OpenUrl availability

Full text

Cite this

Chen, C., Jost, M., Clark, B., Martin, M., Matny, O., Steffenson, B. J., Franckowiak, J. D., Mascher, M., Singh, D., Perovic, D., Richardson, T., Periyannan, S., Lagudah, E. S., Park, R. F., & Dracatos, P. M. (2021). BED domain-containing NLR from wild barley confers resistance to leaf rust. Plant Biotechnology Journal, 19(6), 1206-1215. https://doi.org/10.1111/pbi.13542

@article{64413cdd13664dd9bfbb0fd09501d6ac,

title = "BED domain-containing NLR from wild barley confers resistance to leaf rust",

abstract = "Leaf rust, caused by Puccinia hordei, is a devastating fungal disease affecting barley (Hordeum vulgare subsp. vulgare) production globally. Despite the effectiveness of genetic resistance, the deployment of single genes often compromises durability due to the emergence of virulent P. hordei races, prompting the search for new sources of resistance. Here we report on the cloning of Rph15, a resistance gene derived from barley{\textquoteright}s wild progenitor H. vulgare subsp. spontaneum. We demonstrate using introgression mapping, mutation and complementation that the Rph15 gene from the near-isogenic line (NIL) Bowman + Rph15 (referred to as BW719) encodes a coiled-coil nucleotide-binding leucine-rich repeat (NLR) protein with an integrated Zinc finger BED (ZF-BED) domain. A predicted KASP marker was developed and validated across a collection of Australian cultivars and a series of introgression lines in the Bowman background known to carry the Rph15 resistance. Rph16 from HS-680, another wild barley derived leaf rust resistance gene, was previously mapped to the same genomic region on chromosome 2H and was assumed to be allelic with Rph15 based on genetic studies. Both sequence analysis, race specificity and the identification of a knockout mutant in the HS-680 background suggest that Rph15- and Rph16-mediated resistances are in fact the same and not allelic as previously thought. The cloning of Rph15 now permits efficient gene deployment and the production of resistance gene cassettes for sustained leaf rust control.",

keywords = "Gene cloning, NLR, leaf rust resistance, wild barley",

author = "Chunhong Chen and Matthias Jost and Bethany Clark and Matthew Martin and Oadi Matny and Steffenson, {Brian J.} and Franckowiak, {Jerome D.} and Martin Mascher and Davinder Singh and Dragan Perovic and Terese Richardson and Sambasivam Periyannan and Lagudah, {Evans S.} and Park, {Robert F.} and Dracatos, {Peter M.}",

note = "Funding Information: This work was supported by funds provided through Grains and Development Corporation (US00074), Judith and David Coffey and family and the Lieberman-Okinow Endowment at the University of Minnesota. We are grateful to Matthew Williams, Dorian Friendship (University of Sydney), Smitha Louis and Dhara Bhatt (CSIRO Agriculture and Food, Australia) for technical assistance. The authors also thank Dr. Peter Dodds and Dr. Michael Ayliffe for commenting on the manuscript. Funding Information: This work was supported by funds provided through Grains and Development Corporation (US00074), Judith and David Coffey and family and the Lieberman‐Okinow Endowment at the University of Minnesota. We are grateful to Matthew Williams, Dorian Friendship (University of Sydney), Smitha Louis and Dhara Bhatt (CSIRO Agriculture and Food, Australia) for technical assistance. The authors also thank Dr. Peter Dodds and Dr. Michael Ayliffe for commenting on the manuscript. Publisher Copyright: {\textcopyright} 2021 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.",

year = "2021",

month = jun,

doi = "10.1111/pbi.13542",

language = "English (US)",

volume = "19",

pages = "1206--1215",

journal = "Plant Biotechnology Journal",

issn = "1467-7644",

publisher = "Wiley-Blackwell",

number = "6",

}

TY - JOUR

T1 - BED domain-containing NLR from wild barley confers resistance to leaf rust

AU - Chen, Chunhong

AU - Jost, Matthias

AU - Clark, Bethany

AU - Martin, Matthew

AU - Matny, Oadi

AU - Steffenson, Brian J.

AU - Franckowiak, Jerome D.

AU - Mascher, Martin

AU - Singh, Davinder

AU - Perovic, Dragan

AU - Richardson, Terese

AU - Periyannan, Sambasivam

AU - Lagudah, Evans S.

AU - Park, Robert F.

AU - Dracatos, Peter M.

N1 - Funding Information: This work was supported by funds provided through Grains and Development Corporation (US00074), Judith and David Coffey and family and the Lieberman-Okinow Endowment at the University of Minnesota. We are grateful to Matthew Williams, Dorian Friendship (University of Sydney), Smitha Louis and Dhara Bhatt (CSIRO Agriculture and Food, Australia) for technical assistance. The authors also thank Dr. Peter Dodds and Dr. Michael Ayliffe for commenting on the manuscript. Funding Information: This work was supported by funds provided through Grains and Development Corporation (US00074), Judith and David Coffey and family and the Lieberman‐Okinow Endowment at the University of Minnesota. We are grateful to Matthew Williams, Dorian Friendship (University of Sydney), Smitha Louis and Dhara Bhatt (CSIRO Agriculture and Food, Australia) for technical assistance. The authors also thank Dr. Peter Dodds and Dr. Michael Ayliffe for commenting on the manuscript. Publisher Copyright: © 2021 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

PY - 2021/6

Y1 - 2021/6

N2 - Leaf rust, caused by Puccinia hordei, is a devastating fungal disease affecting barley (Hordeum vulgare subsp. vulgare) production globally. Despite the effectiveness of genetic resistance, the deployment of single genes often compromises durability due to the emergence of virulent P. hordei races, prompting the search for new sources of resistance. Here we report on the cloning of Rph15, a resistance gene derived from barley’s wild progenitor H. vulgare subsp. spontaneum. We demonstrate using introgression mapping, mutation and complementation that the Rph15 gene from the near-isogenic line (NIL) Bowman + Rph15 (referred to as BW719) encodes a coiled-coil nucleotide-binding leucine-rich repeat (NLR) protein with an integrated Zinc finger BED (ZF-BED) domain. A predicted KASP marker was developed and validated across a collection of Australian cultivars and a series of introgression lines in the Bowman background known to carry the Rph15 resistance. Rph16 from HS-680, another wild barley derived leaf rust resistance gene, was previously mapped to the same genomic region on chromosome 2H and was assumed to be allelic with Rph15 based on genetic studies. Both sequence analysis, race specificity and the identification of a knockout mutant in the HS-680 background suggest that Rph15- and Rph16-mediated resistances are in fact the same and not allelic as previously thought. The cloning of Rph15 now permits efficient gene deployment and the production of resistance gene cassettes for sustained leaf rust control.

AB - Leaf rust, caused by Puccinia hordei, is a devastating fungal disease affecting barley (Hordeum vulgare subsp. vulgare) production globally. Despite the effectiveness of genetic resistance, the deployment of single genes often compromises durability due to the emergence of virulent P. hordei races, prompting the search for new sources of resistance. Here we report on the cloning of Rph15, a resistance gene derived from barley’s wild progenitor H. vulgare subsp. spontaneum. We demonstrate using introgression mapping, mutation and complementation that the Rph15 gene from the near-isogenic line (NIL) Bowman + Rph15 (referred to as BW719) encodes a coiled-coil nucleotide-binding leucine-rich repeat (NLR) protein with an integrated Zinc finger BED (ZF-BED) domain. A predicted KASP marker was developed and validated across a collection of Australian cultivars and a series of introgression lines in the Bowman background known to carry the Rph15 resistance. Rph16 from HS-680, another wild barley derived leaf rust resistance gene, was previously mapped to the same genomic region on chromosome 2H and was assumed to be allelic with Rph15 based on genetic studies. Both sequence analysis, race specificity and the identification of a knockout mutant in the HS-680 background suggest that Rph15- and Rph16-mediated resistances are in fact the same and not allelic as previously thought. The cloning of Rph15 now permits efficient gene deployment and the production of resistance gene cassettes for sustained leaf rust control.

KW - Gene cloning

KW - NLR

KW - leaf rust resistance

KW - wild barley

UR - http://www.scopus.com/inward/record.url?scp=85102057797&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85102057797&partnerID=8YFLogxK

U2 - 10.1111/pbi.13542

DO - 10.1111/pbi.13542

M3 - Article

C2 - 33415836

AN - SCOPUS:85102057797

SN - 1467-7644

VL - 19

SP - 1206

EP - 1215

JO - Plant Biotechnology Journal

JF - Plant Biotechnology Journal

IS - 6

ER -

BED domain-containing NLR from wild barley confers resistance to leaf rust

Abstract

Bibliographical note

Keywords

PubMed: MeSH publication types

UN SDGs

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this