TY - JOUR
T1 - Binding of serotonergic ligands to brain membranes of alcohol-preferring AA and alcohol-avoiding ANA rats
AU - Korpi, E. R.
AU - Päivärinta, P.
AU - Abi-Dargham, A.
AU - Honkanen, A.
AU - Laruelle, M.
AU - Tuominen, K.
AU - Hilakivi, L. A.
PY - 1992
Y1 - 1992
N2 - The alcohol-preferring AA rats have higher concentration of 5-hydroxytryptamine (5-HT) in the brain than the alcohol-avoiding ANA rats. In the present study, the 5-HT1, 5-HT2, and 5-HT3 receptors were studied with [3H]5-HT, [3H]ketanserin, and [3H]LY278584, respectively, in membrane homogenates from different brain regions of both rat lines using in vitro binding assays. No differences in the 5-HT1 and 5-HT2 receptor binding in the brainstem, hippocampus, frontal cortex, and hypothalamus or in the 5-HT3 receptor binding in the nucleus accumbens, amygdala, hippocampus, and frontal cortex were observed between the ethanol-naive animals of the rat lines. In rats given the opportunity to voluntarily consume alcohol, there was a tendency to increase 5-HT1 binding in the ANA rats, which tendency was, however, also found in their ethanol-naive controls subjected to the same handling and behavioral tests as the ethanol-experienced animals. The results do not, however, indicate that any genetic modifications of the 5-HT receptor-binding sites have occurred in the process of the selective breeding of AA and ANA rats for alcohol preference and avoidance, respectively.
AB - The alcohol-preferring AA rats have higher concentration of 5-hydroxytryptamine (5-HT) in the brain than the alcohol-avoiding ANA rats. In the present study, the 5-HT1, 5-HT2, and 5-HT3 receptors were studied with [3H]5-HT, [3H]ketanserin, and [3H]LY278584, respectively, in membrane homogenates from different brain regions of both rat lines using in vitro binding assays. No differences in the 5-HT1 and 5-HT2 receptor binding in the brainstem, hippocampus, frontal cortex, and hypothalamus or in the 5-HT3 receptor binding in the nucleus accumbens, amygdala, hippocampus, and frontal cortex were observed between the ethanol-naive animals of the rat lines. In rats given the opportunity to voluntarily consume alcohol, there was a tendency to increase 5-HT1 binding in the ANA rats, which tendency was, however, also found in their ethanol-naive controls subjected to the same handling and behavioral tests as the ethanol-experienced animals. The results do not, however, indicate that any genetic modifications of the 5-HT receptor-binding sites have occurred in the process of the selective breeding of AA and ANA rats for alcohol preference and avoidance, respectively.
KW - 5-HT receptors
KW - AA and ANA rats
KW - Selective breeding
KW - Voluntary alcohol consumption
KW - [H]5-HT
KW - [H]ketanserin
KW - [H]LY278584
UR - http://www.scopus.com/inward/record.url?scp=0026745076&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026745076&partnerID=8YFLogxK
U2 - 10.1016/0741-8329(92)90034-8
DO - 10.1016/0741-8329(92)90034-8
M3 - Article
C2 - 1418660
AN - SCOPUS:0026745076
SN - 0741-8329
VL - 9
SP - 369
EP - 374
JO - Alcohol
JF - Alcohol
IS - 5
ER -