Bioactivity of freeze-dried platelet-rich plasma in an adsorbed form on a biodegradable polymer material

Yu Nakajima, Tomoyuki Kawase, Mito Kobayashi, Kazuhiro Okuda, Larry F. Wolff, Hiromasa Yoshie

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


Owing to the necessity for the immediate preparation from patients' blood, autologous platelet-rich plasma (PRP) limits its clinical applicability. To address this concern and respond to emergency care and other unpredictable uses, we have developed a freeze-dried PRP in an adsorbed form on a biodegradable polymer material (Polyglactin 910). On the polymer filaments of PRP mesh, which was prepared by coating the polymer mesh with human fresh PRP and subsequent freeze-drying, platelets were incorporated, and related growth factors were preserved at high levels. This new PRP mesh preparation significantly and reproducibly stimulated the proliferation of human periodontal ligament cells in vitro and neovascularization in a chorioallantoic membrane assay. A full-thickness skin defect model in a diabetic mouse demonstrated the PRP mesh, although prepared from human blood, substantially facilitated angiogenesis, granulation tissue formation, and re-epithelialization without inducing severe inflammation in vivo. These data demonstrate that our new PRP mesh preparation functions as a bioactive material to facilitate tissue repair/regeneration. Therefore, we suggest that this bioactive material, composed of allogeneic PRP, could be clinically used as a promising alternative in emergency care or at times when autologous PRP is not prepared immediately before application.

Original languageEnglish (US)
Pages (from-to)594-603
Number of pages10
Issue number8
StatePublished - Dec 2012

Bibliographical note

Funding Information:
Declaration of interests: This project was funded through support by a Grant-in-Aid for Scientific Research from the Ministry of Education, Sports, Science, and Technology, Japan, and by Practical Application research from the Japan Science and Technology Agency. The authors declare they have no competing interests.


  • Angiogenesis
  • Freeze-dry
  • Growth factors
  • Platelet-rich plasma
  • Wound healing

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