Biodistribution of TNF-α-coated gold nanoparticles in an in vivo model system

Raghav Goel, Neha Shah, Rachana Visaria, Giulio F. Paciotti, John C. Bischof

Research output: Contribution to journalArticlepeer-review

147 Scopus citations

Abstract

Aim: In this study, we describe the biodistribution of CYT-6091, a colloidal gold (Au)-based nanomedicine that targets the delivery of TNF-α to solid tumors. Materials & Methods: A single intravenous injection of CYT-6091 coated with 5 μg TNF-α was given to human prostate tumor-bearing or naive (without tumor) nude mice. Tissues were harvested and analyzed at specific time points for Au nanoparticles by atomic emission spectroscopy and TNF-α by ELISA. Results: The two constituents of CYT-6091, TNF-α and Au, exhibited different behavior in blood, with TNF-α showing a faster decay than the Au nanoparticles. Between 0 and 4 h after injection, TNF-α showed a preferential accumulation in the tumor. Au was observed to accumulate preferentially in the liver between 4 and 12 h, and showed some clearance over time (4 months). Conclusion: These data suggest that CYT-6091 delivers TNF-α preferentially to the tumor and that upon TNF-α degradation, the liver takes up Au, which is cleared slowly over time.

Original languageEnglish (US)
Pages (from-to)401-410
Number of pages10
JournalNanomedicine
Volume4
Issue number4
DOIs
StatePublished - 2009

Keywords

  • Biodistribution
  • CYT-6091
  • Cryosurgery
  • Gold nanoparticle
  • TNF

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