TY - JOUR
T1 - Biofilm dissolution and cleaning ability of different irrigant solutions on intraorally infected dentin
AU - Del Carpio-Perochena, Aldo E.
AU - Bramante, Clovis M.
AU - Duarte, Marco A.H.
AU - Cavenago, Bruno Cavalini
AU - Villas-Boas, Marcelo H.
AU - Graeff, Marcia S.
AU - Bernardineli, Norberti
AU - De Andrade, Flaviana Bombarda
AU - Ordinola-Zapata, Ronald
N1 - Funding Information:
This work was supported by FAPESP process 2009/03217-5, 2010/16002-4 .
Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2011/8
Y1 - 2011/8
N2 - Introduction: The aim of this study was to evaluate the biofilm dissolution and cleaning ability of different irrigant solutions on intraorally infected dentin. Methods: One hundred twenty bovine dentin specimens were infected intraorally by using a removable orthodontic device. Thirty samples were used for each irrigant solution: 2% chlorhexidine and 1%, 2.5%, and 5.25% sodium hypochlorite (NaOCl). The solutions were used for 5, 15, and 30 minutes and at 2 experimental volumes, 500 μL and 1 mL. The samples were stained by using acridine orange dye before and after the experiments and evaluated by using a confocal microscope. The percentage of biofilm, isolated cells, and noncolonized dentin was measured by using a grid system. Differences in the reduction or increase of the studied parameters were assessed by using nonparametric methods (P <.05). Results: The higher values of biofilm dissolution and noncolonized dentin were found in the 30-minute NaOCl group and in the 5-minute and 15-minute groups of 5.25% NaOCL. The use of 2% chlorhexidine solution did not improve the biofilm dissolution or increase the cleaning of the dentin in comparison with the NaOCl solutions (P <.05). Conclusions: Two percent chlorhexidine does not dissolve the biofilms. Thirty minutes of NaOCl are necessary to have higher values of biofilm dissolution and to increase the cleaning of the dentin independently of the concentration in comparison with the 5-minute and 15-minute contact times.
AB - Introduction: The aim of this study was to evaluate the biofilm dissolution and cleaning ability of different irrigant solutions on intraorally infected dentin. Methods: One hundred twenty bovine dentin specimens were infected intraorally by using a removable orthodontic device. Thirty samples were used for each irrigant solution: 2% chlorhexidine and 1%, 2.5%, and 5.25% sodium hypochlorite (NaOCl). The solutions were used for 5, 15, and 30 minutes and at 2 experimental volumes, 500 μL and 1 mL. The samples were stained by using acridine orange dye before and after the experiments and evaluated by using a confocal microscope. The percentage of biofilm, isolated cells, and noncolonized dentin was measured by using a grid system. Differences in the reduction or increase of the studied parameters were assessed by using nonparametric methods (P <.05). Results: The higher values of biofilm dissolution and noncolonized dentin were found in the 30-minute NaOCl group and in the 5-minute and 15-minute groups of 5.25% NaOCL. The use of 2% chlorhexidine solution did not improve the biofilm dissolution or increase the cleaning of the dentin in comparison with the NaOCl solutions (P <.05). Conclusions: Two percent chlorhexidine does not dissolve the biofilms. Thirty minutes of NaOCl are necessary to have higher values of biofilm dissolution and to increase the cleaning of the dentin independently of the concentration in comparison with the 5-minute and 15-minute contact times.
KW - Acridine orange
KW - bacteria
KW - confocal laser scanning microscopy
KW - dentin
KW - irrigant solutions
KW - oral biofilms
UR - http://www.scopus.com/inward/record.url?scp=80955180578&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80955180578&partnerID=8YFLogxK
U2 - 10.1016/j.joen.2011.04.013
DO - 10.1016/j.joen.2011.04.013
M3 - Article
C2 - 21763908
AN - SCOPUS:80955180578
SN - 0099-2399
VL - 37
SP - 1134
EP - 1138
JO - Journal of Endodontics
JF - Journal of Endodontics
IS - 8
ER -