TY - JOUR
T1 - Biomarkers of folate status in NHANES
T2 - A roundtable summary1-6
AU - Yetley, Elizabeth A.
AU - Pfeiffer, Christine M.
AU - Phinney, Karen W.
AU - Fazili, Zia
AU - Lacher, David A.
AU - Bailey, Regan L.
AU - Blackmore, Sheena
AU - Bock, Jay L.
AU - Brody, Lawrence C.
AU - Carmel, Ralph
AU - Curtin, L. Randy
AU - Durazo-Arvizu, Ramón A.
AU - Eckfeldt, John H
AU - Green, Ralph
AU - Gregory, Jesse F.
AU - Hoofnagle, Andrew N.
AU - Jacobsen, Donald W.
AU - Jacques, Paul F.
AU - Molloy, Anne M.
AU - Massaro, Joseph
AU - Mills, James L.
AU - Nexo, Ebba
AU - Rader, Jeanne I.
AU - Selhub, Jacob
AU - Sempos, Christopher
AU - Shane, Barry
AU - Stabler, Sally
AU - Stover, Patrick
AU - Tamura, Tsunenobu
AU - Tedstone, Alison
AU - Thorpe, Susan J.
AU - Coates, Paul M.
AU - Johnson, Clifford L.
AU - Picciano, Mary Frances
PY - 2011/7/1
Y1 - 2011/7/1
N2 - A roundtable to discuss the measurement of folate status biomarkers in NHANES took place in July 2010. NHANES has measured serum folate since 1974 and red blood cell (RBC) folate since 1978 with the use of several different measurement procedures. Data on serum 5-methyltetrahydrofolate (5MTHF) and folic acid (FA) concentrations in persons aged ≥60 y are available in NHANES 1999-2002. The roundtable reviewed data that showed that folate concentrations from the Bio-Rad Quantaphase II procedure (Bio-Rad Laboratories, Hercules, CA; used in NHANES 1991-1994 and NHANES 1999-2006) were, on average, 29% lower for serum and 45% lower for RBC than were those from the microbiological assay (MA), which was used in NHANES 2007-2010. Roundtable experts agreed that these differences required a data adjustment for time-trend analyses. The roundtable reviewed the possible use of an isotope-dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS) measurement procedure for future NHANES and agreed that the close agreement between the MA and LC-MS/MS results for serum folate supported conversion to the LC-MS/MS procedure. However, for RBC folate, the MA gave 25% higher concentrations than did the LC-MS/MS procedure. The roundtable agreed that the use of the LC-MS/MS procedure to measure RBC folate is premature at this time. The roundtable reviewed the reference materials available or under development at the National Institute of Standards and Technology and recognized the challenges related to, and the scientific need for, these materials. They noted the need for a commutability study for the available reference materials for serum 5MTHF and FA.
AB - A roundtable to discuss the measurement of folate status biomarkers in NHANES took place in July 2010. NHANES has measured serum folate since 1974 and red blood cell (RBC) folate since 1978 with the use of several different measurement procedures. Data on serum 5-methyltetrahydrofolate (5MTHF) and folic acid (FA) concentrations in persons aged ≥60 y are available in NHANES 1999-2002. The roundtable reviewed data that showed that folate concentrations from the Bio-Rad Quantaphase II procedure (Bio-Rad Laboratories, Hercules, CA; used in NHANES 1991-1994 and NHANES 1999-2006) were, on average, 29% lower for serum and 45% lower for RBC than were those from the microbiological assay (MA), which was used in NHANES 2007-2010. Roundtable experts agreed that these differences required a data adjustment for time-trend analyses. The roundtable reviewed the possible use of an isotope-dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS) measurement procedure for future NHANES and agreed that the close agreement between the MA and LC-MS/MS results for serum folate supported conversion to the LC-MS/MS procedure. However, for RBC folate, the MA gave 25% higher concentrations than did the LC-MS/MS procedure. The roundtable agreed that the use of the LC-MS/MS procedure to measure RBC folate is premature at this time. The roundtable reviewed the reference materials available or under development at the National Institute of Standards and Technology and recognized the challenges related to, and the scientific need for, these materials. They noted the need for a commutability study for the available reference materials for serum 5MTHF and FA.
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U2 - 10.3945/ajcn.111.013011
DO - 10.3945/ajcn.111.013011
M3 - Article
C2 - 21593502
AN - SCOPUS:79959488043
SN - 0002-9165
VL - 94
SP - 303S-312S
JO - American Journal of Clinical Nutrition
JF - American Journal of Clinical Nutrition
IS - 1
ER -