Blocking primers reduce co-amplification of plant DNA when studying bacterial endophyte communities

Brett E Arenz; Dan C. Schlatter; Jim Bradeen; Linda L Kinkel

doi:10.1016/j.mimet.2015.07.003

Blocking primers reduce co-amplification of plant DNA when studying bacterial endophyte communities

Brett E Arenz, Dan C. Schlatter, Jim Bradeen, Linda L Kinkel

Plant Pathology

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

A blocking primer set based on the technique described by Vestheim and Jarman (2008) was developed to reduce amplification of non-target plant DNA when conducting metagenomic studies on bacterial endophyte communities. Bacterial amplification efficiency was increased 300-fold compared to standard PCR in an Illumina-based study of Sorghastrum nutans leaves.

Original language	English (US)
Pages (from-to)	1-3
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	117
DOIs	https://doi.org/10.1016/j.mimet.2015.07.003
State	Published - Oct 1 2015

Bibliographical note

Funding Information:
We thank Chistopher Staley of the University of Minnesota for sharing standard bacterial 16S Illumina primers. This material is based upon work that is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award number 2011 67019–30330 and the University of Minnesota Agricultural Experiment Station (Project MIN 22-018).

Keywords

Blocking
Chloroplast
Endophyte
Metagenomics
Mitochondria

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title = "Blocking primers reduce co-amplification of plant DNA when studying bacterial endophyte communities",

abstract = "A blocking primer set based on the technique described by Vestheim and Jarman (2008) was developed to reduce amplification of non-target plant DNA when conducting metagenomic studies on bacterial endophyte communities. Bacterial amplification efficiency was increased 300-fold compared to standard PCR in an Illumina-based study of Sorghastrum nutans leaves.",

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AU - Kinkel, Linda L

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KW - Mitochondria

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Blocking primers reduce co-amplification of plant DNA when studying bacterial endophyte communities

Abstract

Bibliographical note

Keywords

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